Effect of tributyltin exposure on the embryonic development and behavior of a molluscan model species, Lymnaea stagnalis

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology Vol. 285; p. 109996
• Main Authors: Svigruha, Réka, Molnár, László, Elekes, Károly, Pirger, Zsolt, Fodor, István
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2024
• Subjects: Animals; Behavior, Animal - drug effects; Development; Embryo, Nonmammalian - drug effects; Embryonic Development - drug effects; Feeding; Feeding Behavior - drug effects; Heart rate; Heart Rate - drug effects; Locomotion; Locomotion - drug effects; Lymnaea - drug effects; Lymnaea - embryology; Lymnaea stagnalis; Mollusk; Tin accumulation; Trialkyltin Compounds - toxicity; Tributyltin; Water Pollutants, Chemical - toxicity; Lymnaea stagnalis; Heart rate; Locomotion; Tin accumulation; Mollusk; Development; Tributyltin; Feeding
• ISSN: 1532-0456
• DOI: 10.1016/j.cbpc.2024.109996

The presence of the organotin compound tributyltin (TBT) in aquatic ecosystems has been a serious environmental problem for decades. Although a number of studies described the negative impact of TBT on mollusks at different levels, investigations connected to its potential effects during embryogenesis have been neglected. For a better understanding of the impact of TBT on mollusks, in the present study, embryos of previously TBT-treated or not treated specimens of the great pond snail (Lymnaea stagnalis) were exposed to 100 ng L−1 TBT from egg-laying (single-cell stage) until hatching. According to our results, TBT significantly delayed hatching and caused shell malformation. TBT transiently decreased the locomotion (gliding) and also reduced the feeding activity, demonstrating for the first time that this compound can alter the behavioral patterns of molluscan embryos. The heart rate was also significantly reduced, providing further support that cardiac activity is an excellent indicator of metal pollution in molluscan species. At the histochemical level, tin was demonstrated for the first time in TBT-treated hatchlings with intensive reaction in the central nervous system, kidney, and hepatopancreas. Overall, the most notable effects were observed in treated embryos derived from TBT treated snails. Our findings indicate that TBT has detrimental effects on the development and physiological functions of Lymnaea embryos even at a sub-lethal concentration, potentially influencing their survival and fitness. Highlighting our observations, we have demonstrated previously unknown physiological changes (altered heart rate, locomotion, and feeding activity) caused by TBT, as well as visualized tin at the histochemical level in a molluscan species for the first time following TBT exposure. Further studies are in progress to reveal the cellular and molecular mechanisms underlying the physiological and behavioral changes described in the present study. [Display omitted] •The effect of 100 ng L−1 TBT on Lymnaea embryos was investigated.•TBT significantly delayed hatching and caused shell malformation.•Decrease in heart rate, gliding, and feeding were observed.•Tin accumulated in the nervous system, kidney, and hepatopancreas of hatchlings.