A bovine glucuronidase for assembly of beta-D-glucuronyl-(1-3)-6-O-sulfo-beta-D-gluco- and galacto-pyranosyl linkages

Glucuronidase-catalyzed transglycosylation was examined by using 4-nitrophenyl beta-D-glucuronide ((D)-GlcA-O-pNP) as the glycosyl donor; when pNP 6-O-sulfo-beta-(D)-gluco-and D-galacto-pyranosides were used as the acceptors, a bovine enzyme was found to construct beta-(D)-GlcA-(1-3)-linkages with t...

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Bibliographic Details
Published inChemical communications (Cambridge, England) no. 13; pp. 1381 - 1383
Main Authors Uzawa, H, Nagatsuka, T, Hiramatsu, H, Nishida, Y
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 01.01.2006
Subjects
Animals
Catalysis
Cattle
Chemistry
Chemistry, Multidisciplinary
Disaccharides - chemistry
Disaccharides - metabolism
Glucuronidase - metabolism
Glycosylation
Molecular Structure
Physical Sciences
Science & Technology
PRION PROTEIN
HIGH-AFFINITY
ANTITHROMBIN-III
OLIGOSACCHARIDES
SYNTHETIC APPROACH
HEPARIN PENTASACCHARIDE FRAGMENT
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Summary:Glucuronidase-catalyzed transglycosylation was examined by using 4-nitrophenyl beta-D-glucuronide ((D)-GlcA-O-pNP) as the glycosyl donor; when pNP 6-O-sulfo-beta-(D)-gluco-and D-galacto-pyranosides were used as the acceptors, a bovine enzyme was found to construct beta-(D)-GlcA-(1-3)-linkages with the 6-O-sulfosugars in both a site-and beta-selective way.
ISSN:1359-7345
1364-548X
DOI:10.1039/b516921f