Deletion of lncRNA5512 has no effect on spermatogenesis and reproduction in mice

Long non-coding (lnc) RNAs are a series of RNAs longer than 200 nucleotides that do not code for protein products. Whole-genome expression profiles of lncRNAs suggest that they play important roles in spermatogenesis because they are particularly abundant in testes. However, most of their characteri...

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Bibliographic Details
Published inReproduction fertility and development Vol. 32; no. 7; p. 706
Main Authors Zhu, Yu, Lin, Yu, He, Yue, Wang, Hanshu, Chen, Shitao, Li, Zhenhua, Song, Ning, Sun, Fei
Format Journal Article
LanguageEnglish
Published Australia 01.01.2020
Subjects
Animals
CRISPR-Associated Protein 9
Epididymis - chemistry
Epididymis - physiology
Female
Inverted Repeat Sequences
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Reproduction - genetics
Reproduction - physiology
RNA, Long Noncoding - analysis
RNA, Long Noncoding - genetics
RNA, Long Noncoding - physiology
Semen Analysis
Spermatogenesis - genetics
Spermatogenesis - physiology
Spermatozoa - chemistry
Spermatozoa - physiology
Testis - chemistry
Testis - physiology
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Summary:Long non-coding (lnc) RNAs are a series of RNAs longer than 200 nucleotides that do not code for protein products. Whole-genome expression profiles of lncRNAs suggest that they play important roles in spermatogenesis because they are particularly abundant in testes. However, most of their characteristics and functions remain unclear. The aim of this study was to define the function of lncRNA5512, which is abundant in spermatocytes and round spermatids, in mouse fertility invivo. To investigate this we generated lncRNA5512-knockout mice by clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 technology. Knockout mice showed normal spermatogenesis and fertility, and had no detectable abnormalities. This indicates that lncRNA5512 does not affect mouse fertility despite its high expression in the testes. Its specific localisation in spermatocytes and round spermatids suggests that it could be a useful marker for the identification of spermatocytes and round spermatids in mouse testes.
ISSN:1031-3613
DOI:10.1071/RD19246