A Human Promyelocyte mRNA Transiently Induced by TPA Is Homologous to Yeast IPP Isomerase

A cDNA clone was isolated from HL60 human promyelocyte cells on the basis of the induction of its cognate mRNA by the phorbol ester TPA (12- 0-tetradecanoylphorbol-13-acetate) in the presence of cycloheximide. Northern analysis showed a peak of induction of a 1.9-kb mRNA at 3-6 h after TPA addition....

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Bibliographic Details
Published inGenomics (San Diego, Calif.) Vol. 20; no. 1; pp. 129 - 131
Main Authors Xuan, Jian-Wu, Kowalski, Jacek, Chambers, Ann F., Denhardt, David T.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.03.1994
Subjects
Amino Acid Sequence
Base Sequence
BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
Carbon-Carbon Double Bond Isomerases
Cell Line
CLONING
DIENES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA, Complementary - genetics
DNA-CLONING
ENZYMES
GENES
Granulocytes - drug effects
Granulocytes - metabolism
Humans
HYBRIDIZATION
HYDROCARBONS
ISOMERASES
Isomerases - genetics
Molecular Sequence Data
ORGANIC COMPOUNDS
POLYENES
PROTEINS
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Sequence Homology, Amino Acid
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS 550400 > Genetics
Tetradecanoylphorbol Acetate - pharmacology
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Summary:A cDNA clone was isolated from HL60 human promyelocyte cells on the basis of the induction of its cognate mRNA by the phorbol ester TPA (12- 0-tetradecanoylphorbol-13-acetate) in the presence of cycloheximide. Northern analysis showed a peak of induction of a 1.9-kb mRNA at 3-6 h after TPA addition. Sequence analysis revealed that this cDNA was likely a human homolog of yeast isopentenyl diphosphate:dimethylallyl diphosphate isomerase, an enzyme important for isoprenoid synthesis and the prenylation of proteins such as ras p21.
Bibliography:None
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1994.1139