Chlamydia trachomatis from men with non-gonococcal urethritis. Simplified procedure for cultivation and isolation in replicating McCoy cell culture

Chlamydia trachromatis was cultivated on replicating McCoy cells without the addition of antimetabolites. A further technical modification was centrifugation of the specimens at room temperature at 4000 g, thus making it possible to use the method in any microbiological laboratory. C. trachomatis wa...

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Bibliographic Details
Published inActa pathologica et microbiologica Scandinavica. Section B, Microbiology Vol. 86; no. 4; p. 257
Main Author Csángó, P A
Format Journal Article
LanguageEnglish
Published Denmark 01.09.1978
Subjects
Adult
Bacteriological Techniques
Chlamydia Infections - microbiology
Chlamydia trachomatis - isolation & purification
Humans
Male
Urethritis - microbiology
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Summary:Chlamydia trachromatis was cultivated on replicating McCoy cells without the addition of antimetabolites. A further technical modification was centrifugation of the specimens at room temperature at 4000 g, thus making it possible to use the method in any microbiological laboratory. C. trachomatis was isolated from 36 of 81 patients (44.4%) with non-gonococcal urethritis. This rate compares well with reported isolation rates using antireplicative agents and higher centrifugation temperatures.
ISSN:0105-0656
DOI:10.1111/j.1699-0463.1978.tb00041.x