Behavior of lysozyme adsorbed onto biological liquid crystal lipid monolayer at the air/water interface
The interaction between proteins and lipids is one of the basic problems of modern biochemistry and biophysics.The purpose of this study is to compare the penetration degree of lysozyme into 1,2-diapalmitoyl-sn-glycero-3-phosphocholine（DPPC） and 1,2-dipalmitoyl-sn-glycero-3-phosphoethano-lamine（DPPE...
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| Published in | Chinese physics B Vol. 25; no. 9; pp. 49 - 55 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
01.09.2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-1056 2058-3834 |
| DOI | 10.1088/1674-1056/25/9/090506 |
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| Summary: | The interaction between proteins and lipids is one of the basic problems of modern biochemistry and biophysics.The purpose of this study is to compare the penetration degree of lysozyme into 1,2-diapalmitoyl-sn-glycero-3-phosphocholine（DPPC） and 1,2-dipalmitoyl-sn-glycero-3-phosphoethano-lamine（DPPE） by analyzing the data of surface pressure–area（π–A） isotherms and surface pressure–time（π–T） curves.Lysozyme can penetrate into both DPPC and DPPE monolayers because of the increase of surface pressure at an initial pressure of 15 m N/m.However,the changes of DPPE are larger than DPPC,indicating stronger interaction of lysozyme with DPPE than DPPC.The reason may be due to the different head groups and phase state of DPPC and DPPE monolayers at the surface pressure of 15 m N/m.Atomic force microscopy reveals that lysozyme was absorbed by DPPC and DPPE monolayers,which leads to self-aggregation and self-assembly,forming irregular multimers and conical multimeric.Through analysis,we think that the process of polymer formation is similar to the aggregation mechanism of amyloid fibers. |
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| Bibliography: | lysozyme adsorption curves liquid crystal monolayers phase state Xiaolong Lu, Ruixin Shi, Changchun Hao, Huan Chen Lei Zhang, Junhua Li, Guoqing Xu, and Runguang Sun （1 School of Physics and Information Technology, Shaanxi Normal University, Xi＇an 710062, China 2School and Hospital of Stomatology, Jilin University, Changchun 130021, China） 11-5639/O4 The interaction between proteins and lipids is one of the basic problems of modern biochemistry and biophysics.The purpose of this study is to compare the penetration degree of lysozyme into 1,2-diapalmitoyl-sn-glycero-3-phosphocholine（DPPC） and 1,2-dipalmitoyl-sn-glycero-3-phosphoethano-lamine（DPPE） by analyzing the data of surface pressure–area（π–A） isotherms and surface pressure–time（π–T） curves.Lysozyme can penetrate into both DPPC and DPPE monolayers because of the increase of surface pressure at an initial pressure of 15 m N/m.However,the changes of DPPE are larger than DPPC,indicating stronger interaction of lysozyme with DPPE than DPPC.The reason may be due to the different head groups and phase state of DPPC and DPPE monolayers at the surface pressure of 15 m N/m.Atomic force microscopy reveals that lysozyme was absorbed by DPPC and DPPE monolayers,which leads to self-aggregation and self-assembly,forming irregular multimers and conical multimeric.Through analysis,we think that the process of polymer formation is similar to the aggregation mechanism of amyloid fibers. |
| ISSN: | 1674-1056 2058-3834 |
| DOI: | 10.1088/1674-1056/25/9/090506 |