Irradiation of Fucci-expressing HeLa cells using a tapered glass capillary microbeam

• Published in: Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Vol. 348; pp. 127 - 130
• Main Authors: Puttaraksa, Nitipon, Mäckel, Volkhard, Kobayashi, Tomohiro, Kojima, Takao M., Hamagaki, Manabu, Imamoto, Naoko, Yamazaki, Yasunori
• Format: Journal Article
• Language: English
• Published: 01.04.2015
• Subjects: Capillarity; Fluorescence; Glass; Irradiation; Microbeams; Microscopy; Nuclei; Transformations
• ISSN: 0168-583X
• DOI: 10.1016/j.nimb.2014.11.037

Fluorescent, ubiquitination-based cell cycle indicator-expressing HeLa cells have been irradiated with a microbeam set-up at RIKEN for examining the effect to cell cycle after bombarding with protons of 1MeV incident energy. Here, single cells were exposed in their nucleus with different doses (100, 1000, 10,000 and 100,000 protons). Subsequently, the cells were monitored using time-lapse microscopy after irradiation. In this paper, we present the preliminary results for such irradiation for 8 cells. The irradiated cells with up to 10,000 protons showed the change in fluorescence from red to green. This indicates that the transformation from G1 to S/G2 in cell cycle seemed not to be affected by the irradiation up to 10,000 protons. However, the irradiated cell with 100,000 protons died in red fluorescence at 4h after the irradiation.