STAT3 involvement in the acute phase-related expression of the rat haptoglobin gene

• Published in: Molecular biology reports Vol. 27; no. 2; p. 81
• Main Authors: Grigorov, I, Lazić, T, Cvetković, I, Milosavljević, T, Petrović, M
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.06.2000
• Subjects: Acute-Phase Proteins - genetics; Acute-Phase Proteins - metabolism; Acute-Phase Reaction - chemically induced; Acute-Phase Reaction - genetics; Animals; Base Sequence; Binding Sites; Cell Extracts; DNA - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene expression; Haptoglobins - genetics; Haptoglobins - metabolism; Hepatocytes - drug effects; Hepatocytes - metabolism; Liver; Liver - drug effects; Liver - metabolism; Male; Molecular biology; Molecular Sequence Data; Promoter Regions, Genetic; Rats; Rats, Wistar; Rodents; Signal transduction; STAT3 Transcription Factor; Trans-Activators - genetics; Trans-Activators - metabolism; Turpentine - toxicity
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1023/A:1007177605135

Turpentine-induced acute-phase (AP) response in rats is followed by transcriptional activation of the haptoglobin (Hp) gene in liver. Analysing the promoter sequence of the rat Hp gene we postulated an involvement of Signal Transducer and Activator of Transcription 3 (STAT3) in the regulation of this process. Results obtained by using a combination of Western immunoblot and DNA-binding assays revealed AP-induced binding of constitutive 86kD-and inducible 91kD-STAT3 isoforms to the rat Hp gene inducible promoter element. On the basis of these data we assumed that AP-related interactions of these two STAT3 isoforms correlates with an activated transcriptional status of the rat Hp gene.