An efficient transformation method for a potato (Solanum tuberosum L. var. Atlantic)

• Published in: Journal of plant biotechnology Vol. 42; no. 2; pp. 77 - 82
• Main Authors: Han, Eun-Hee, Goo, Young-Min, Lee, Min-Kyung, Lee, Shin-Woo
• Format: Journal Article
• Language: English
• Published: 한국식물생명공학회 01.06.2015
• Subjects: 농학
• ISSN: 1229-2818; 2384-1397
• DOI: 10.5010/JPB.2015.42.2.77

We found that a long period of in vitro culture is a critical factor on the low transformation rate for a specific potato genotype, Solanum tuberosum L. var. Atlantic when phosphinothricin (PPT) was added to select putative transformants in a solid media. The fresh explants of the newly produced plants from a micro-tuber was able to increase the transformation rate significantly while the old explants prepared from a plant maintained for longer than 6 months in vitro by sub-culturing every 3 ~ 4 weeks resulted in a very low transformation frequency. However, Jowon cultivar was not so much influenced by the period of in vitro culture with high transformation rate (higher than 10.0%). Further research need to be explored for the reason why a particular potato genotype, Atlantic is more vulnerable than the Jowon cultivar during the regeneration stage resulting in the low transformation frequency. KCI Citation Count: 0