Anxiolytic Effect of Peptides from Sea Anemone Heteractis crispa, Modulators of TRPV1 and ASIC Channels

• Published in: Doklady. Biochemistry and biophysics Vol. 505; no. 1; pp. 145 - 150
• Main Authors: Sintsova, O. V., Kalina, R. S., Gladkikh, I. N., Palikova, Y. A., Palikov, V. A., Borozdina, N. A., Klimovich, A. A., Menshov, A. S., Dyachenko, I. A., Leychenko, E. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.08.2022; Springer Nature B.V
• Subjects: Acidity; Anxiety; Anxiety disorders; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Capsaicin receptors; Heteractis crispa; Ion channels; Life Sciences; Molecular Biology; Pharmacodynamics; Sodium channels; Transient receptor potential proteins; anxiolytic effect; acid-sensing ion channels; TRPV1 ion channel; analgesic peptides; CNS; antidepressants; anxiety disorders
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672922040093

A number of studies confirmed the involvement of transient receptor potential vanilloid (TRPV) and acid-sensing (ASIC) ion channels in the physiological processes associated with the development of anxiety disorders. This makes their ligands new potential anxiolytic agents. We examined the efficacy of two peptides from the sea anemone Heteractis crispa , Hcr 1b-2 and HCRG21, affecting ASIC1a and TRPV1 channels, respectively, in the open field and elevated plus maze tests. According to the obtained data, HCRG21 significantly decreases both the level of anxiety and stimulates the activity of animals at doses of 0.01–1 mg/kg, whereas Hcr 1b-2 has a weak anxiolytic effect only at a dose of 0.1 mg/kg. The pharmacodynamic study showed that the HCRG21 has an anxiolytic effect for 2 h, and its effectiveness is higher than that of the reference drug.