Clonal analysis of the cytotoxic T-lymphocyte response to reovirus

• Published in: Viral immunology Vol. 4; no. 3; p. 167
• Main Authors: Hogan, K T, Cashdollar, L W
• Format: Journal Article
• Language: English
• Published: United States 1991
• Subjects: Animals; Antigens, Viral - immunology; Cell Line; Colony-Forming Units Assay; Cross Reactions; Cytotoxicity, Immunologic; Epitopes - immunology; Mammalian orthoreovirus 3 - immunology; Mice; Mice, Inbred C3H; Reoviridae - immunology; T-Lymphocytes, Cytotoxic - immunology
• ISSN: 0882-8245
• DOI: 10.1089/vim.1991.4.167

Reovirus has previously been classified into three serotypes based on hemagglutination inhibition assays. In the present study, the specificity of cytotoxic T lymphocytes (CTL) generated in reovirus type 3-infected C3H/HeN mice was investigated at the population and clonal levels. Short-term CTL lines generated in response to reovirus type 3 preferentially lysed cells infected with reovirus type 1 or 3 and, in some instances, type 2-infected cells as well. Eleven CTL clones established from the lines demonstrated two unique patterns of recognition. A single clone was exquisitely specific for reovirus type 3-infected cells and did not cross-react on reovirus type 1- or 2-infected cells. Ten of the clones recognized reovirus type 1- and type 3-infected cells. These clones had low levels of cross-reactivity on reovirus type 2-infected cells that was revealed only at high effector:target cell ratios. Precursor frequency analysis further revealed that the majority of the CTL generated against reovirus type 3 could cross-react on both reovirus type 1- and type 2-infected cells. Some CTL could be detected that had a more restricted pattern of recognition and recognized reovirus type 3-infected cells exclusively or recognized reovirus type 3-infected cells and either reovirus type 1- or type 2-infected cells. These results indicate that a minimum of four epitopes are recognized by reovirus-specific CTL and that the response is dominated by CTL that recognize an epitope common to all three serotypes of reovirus.