Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3
This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH) 2 -D 3 ) of vitamin D 3 . Deficiencies in 1,25-(OH) 2 -D have been linked to renal disease, while elevations are...
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Published in | Analyst (London) Vol. 135; no. 11; pp. 2811 - 2817 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge
Royal Society of Chemistry
01.11.2010
|
Subjects | |
Online Access | Get full text |
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Summary: | This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH)
2
-D
3
) of vitamin D
3
. Deficiencies in 1,25-(OH)
2
-D have been linked to renal disease, while elevations are linked to hypercalcemia. Thus, there has been a sharp increase in the clinical demand for measurements of this metabolite. The work herein extends the many attributes of SERS-based sandwich immunoassays that have been exploited extensively in the detection of large biolytes (
e.g.
, DNA, proteins, viruses, and microorganisms) into a competitive immunoassay for the low level determination of a small biolyte, 1,25-(OH)
2
-D
3
(
M
w
= 416 g mol
−1
). The assay uses surface modified gold nanoparticles as SERS labels, and has a dynamic range of 10-200 pg mL
−1
and a limit of detection of 8.4 ± 1.8 pg mL
−1
. These analytical performance metrics match those of tests for 1,25-(OH)
2
-D
3
that rely on radio- or enzyme-labels, while using a much smaller sample volume and eliminating the disposal of radioactive wastes. Moreover, the SERS-based data from pooled-patient sera show strong agreement with that from radioimmunoassays. The merits and potential utility of this new assay are briefly discussed.
This article highlights the development of a competitive surface-enhanced Raman scattering immunoassay for detection and quantification of 1,25-dihydroxy vitamin D
3
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2654 1364-5528 |
DOI: | 10.1039/c0an00354a |