LCAT-dependent conversion rate is a determinant of plasma preβ1-HDL concentration in healthy Japanese

• Published in: Clinica chimica acta Vol. 350; no. 1; pp. 107 - 114
• Main Authors: Miida, Takashi, Obayashi, Konen, Seino, Utako, Zhu, Ying, Ito, Takako, Kosuge, Keiichiro, Hirayama, Satoshi, Hanyu, Osamu, Nakamura, Yuichi, Yamaguchi, Toshio, Tsuda, Takashi, Saito, Yuji, Miyazaki, Osamu, Nakamura, Yasushi, Okada, Masahiko
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2004
• Subjects: Apolipoprotein AI; HDL; LCAT; Normolipidemia; Prebeta-HDL; Apolipoprotein AI; HDL; LCAT; Prebeta-HDL
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/j.cccn.2004.07.014

Preβ1-HDL acts as a primary acceptor of cellular cholesterol. Preβ1-HDL is converted into α-migrating high-density lipoprotein (HDL) by lecithin/cholesterol acyltransferase (LCAT). We examined whether the LCAT-dependent conversion rate of preβ1-HDL is a determinant of the plasma preβ1-HDL concentration in healthy Japanese. We measured the conversion half time (CHT preβ1), the time required for 50% of baseline preβ1-HDL to be changed into α-migrating HDL by LCAT, in 100 healthy Japanese (47 men, 53 women, 22–88 years). Preβ1-HDL concentration, as determined by immunoassay, was significantly lower in younger women (<50 years, n=24) than in older women (≥50 years, n=29) (16.8±3.3 vs. 21.7±8.0 mg/l apolipoprotein AI (apoAI), p<0.01). There was no significant difference in preβ1-HDL concentration between younger ( n=24) and older ( n=23) men (21.2±6.8 vs. 22.5±6.6 mg/l apoAI). The mean CHT preβ1 for all subjects was 47.4±13.0 min, and was not influenced by gender or age. Preβ1-HDL concentration was positively correlated with CHT preβ1 in both men and women, suggesting that high preβ1-HDL levels may reflect delayed conversion of preβ1-HDL. LCAT-dependent conversion rate is a determinant of plasma preβ1-HDL concentration in healthy Japanese. We speculate that preβ1-HDL concentration may be used as a metabolic marker for HDL maturation.