Recovery of ethylene biosynthesis in diazocyclopentadiene (DACP)-treated tomato fruit

• Published in: Plant growth regulation Vol. 22; no. 2; pp. 73 - 78
• Main Authors: Tian, M.S, Bowen, J.H, Bauchot, A.D, Gong, Y.P, Lallu, N
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.07.1997; Springer Nature B.V
• Subjects: 1-aminocyclopropane-1-carboxylic acid; Agronomy. Soil science and plant productions; Biological and medical sciences; Biosynthesis; color; developmental stages; Economic plant physiology; enzyme activity; ethylene; food composition; Fructification and ripening; Fructification, ripening. Postharvest physiology; Fruits; Fundamental and applied biological sciences. Psychology; Gene expression; genetic regulation; Growth and development; oxidoreductases; Plant physiology and development; postharvest physiology; respiration; ripening; Solanum lycopersicum var. lycopersicum; Tomatoes; Vegetative and sexual reproduction, floral biology, fructification; 1-Aminocyclopropanecarboxylic acid; Fruit; Enzyme; Ethylene; Oxidase; Biosynthesis; Metabolic inhibitor; Postharvest physiology; Ripening; Vegetable crop; Enzymatic activity; Dicotyledones; Angiospermae; Lycopersicon esculentum; Spermatophyta; Oxidoreductases; Solanaceae; Pretreatment; Biological accumulation; Respiration; Coloration; Biological receptor
• ISSN: 0167-6903; 1573-5087
• DOI: 10.1023/A:1005835115937

Diazocyclopentadiene (DACP), a competitive ethylene action inhibitor binds irreversibly to the ethylene receptor to reduce tissue responses to ethylene. Tomato fruit (Lycopersicon esculentum Mill cv 'Rondello') were treated with DACP at the mature green stage. Ethylene biosynthesis and respiration rate were depressed. Color changes from green to red were delayed. Compared to the control, ACC content increased and ACC oxidase activity in vivo decreased in DACP-treated fruit. Thus, decrease of ethylene production caused by DACP treatment was due to the reduction of ACC oxidase activity. The decline in ripening subsequently recovered after DACP treatment. Results from the Northern analysis for gene expression of ACC synthase and ACC oxidase, showed that expression of both genes declined in DACP-treated fruit, and then recovered. Therefore the recovery of ethylene production was due to the recovery in gene expression and activity of ACC oxidase. We conclude that the effects of DACP on ethylene biosynthesis are on expression of ACC synthase and ACC oxidase genes, and/or regulation of ACC oxidase activity.