Effects of seasonal changes on T-helper 1/ T-helper 2 immune balance and eczema onset in rats

• Published in: Journal of Traditional Chinese Medical Sciences Vol. 10; no. 1; pp. 64 - 72
• Main Authors: Zhang, Hairong, Liu, Leilei, Sun, Yike, Li, Wenna, Wang, Ruochong, Sun, Xiao, Zhu, Guangqin, Ma, Shuran
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2023; Elsevier
• Subjects: Eczema; Immunization; Immunoglobulin E; Long summer; Melatonin; Seasonality; Th1/Th2; Th1/Th2; Immunization; Melatonin; Long summer; Eczema; Seasonality; Immunoglobulin E
• ISSN: 2095-7548
• DOI: 10.1016/j.jtcms.2022.11.007

To explore the impact of seasonal changes on the T-helper 1 (Th1)/T-helper 2 (Th2) immune balance of the body in order to elucidate the internal immunological mechanisms underlying seasonal eczema. Eighty 4-week-old male Sprague–Dawley rats were divided into 5 season groups kept in corresponding season simulation environments, and subdivided into the model group and the control group. The Eczema Area and Severity Index (EASI) and scratching frequency were evaluated. The serum levels of immunoglobulin E (IgE), interleukin-2 (IL-2), interleukin-12 (IL-12), and interferon-gamma (IFN-γ), interleukin-4 (IL-4), interleukin-25 (IL-25), and interleukin-31 (IL-31), and melatonin (MT), as well as the MT receptor (MTR) levels in the spleen, were detected by enzyme-linked immunosorbent assay analysis. The mRNA expression levels of T-bet and GATA3 were detected by quantitative real-time polymerase chain reaction. EASI scores and the scratching frequency of the model group were higher in the long summer than in the other 4 seasons. Meanwhile, the serum levels of IgE and the Th2 cytokines IL-4, IL-25, and IL-31, as well as GATA3 mRNA expression levels, were high during the long summer in both groups. However, the serum levels of the Th1 cytokines IL-2, IL-12, and IFN-γ, as well as MT, MTR, and T-bet mRNA levels, were lower during the long summer. In all 5 seasonal groups (spring, summer, long summer, autumn, and winter), the levels of all immune factors, especially IL-4 and IL-31, were higher in the model group than those in the control group, while the concentrations of MT and MTR were lower. Under long light, hot, and humid conditions in the long summer, the body is more likely to suffer from Th2-dominated immune imbalance. This is the internal mechanism behind the high incidence and severity of eczema during the long summer. MT and MTR play a key role in the seasonal onset of eczema.