Nucleotide sequence of canine c-N-ras: codons 1 to 71

The c-N-ras gene has been implicated often in the genesis and/or progression of human leukemias. To our knowledge, the sequence of this gene in the dog has not been reported. Using a system of asymmetric reamplification of double-stranded polymerase chain reaction (PCR) products, we have sequenced n...

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Published inAmerican journal of veterinary research Vol. 53; no. 4; p. 600
Main Authors Saunders, K.A. (University of California, Davis, CA), Madewell, B.R, Oreffo, V.I.C, Kraegel, S.A, Gumerlock, P.H
Format Journal Article
LanguageEnglish
Published United States 01.04.1992
Subjects
Amino Acid Sequence
Animals
Autoradiography
Base Sequence
CARCINOGENOS
CHIEN
Codon - chemistry
DNA - chemistry
Dogs - genetics
GENE
GENES
Genes, ras - genetics
Molecular Sequence Data
Mutation
NEOPLASMAS
NEOPLASME
NUCLEOTIDE
NUCLEOTIDOS
PERRO
Polymerase Chain Reaction
RNA, Messenger - chemistry
Sequence Homology, Nucleic Acid
Species Specificity
SUBSTANCE CANCERIGENE
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Summary:The c-N-ras gene has been implicated often in the genesis and/or progression of human leukemias. To our knowledge, the sequence of this gene in the dog has not been reported. Using a system of asymmetric reamplification of double-stranded polymerase chain reaction (PCR) products, we have sequenced normal canine c-N-ras mRNA from position -26 to +213, including codons 12, 13, and 61, which are the sites where oncogenic mutations are most commonly observed. The canine c-N-ras sequence has close homology with the human sequence in this area; there were only 6 observed base differences in nucleotide sequence and none resulted in a change of the encoded amino acid. The results of this study set the stage for directed searches for c-N-ras mutations in experimentally induced and naturally acquired neoplasms of the dog
Bibliography:L10
9190015
ISSN:0002-9645
1943-5681
DOI:10.2460/ajvr.1991.53.04.600