Circulating Polymorphonuclear Leukocytes from Patients with Gram-Negative Bacteremia Are Not Primed for Enhanced Production of Leukotriene B4 or 5-Hydroxyeicosatetraenoic Acid

• Published in: The Journal of infectious diseases Vol. 169; no. 5; pp. 1151 - 1154
• Main Authors: Sorrell, Tania C., Sztelma, Krystyna, May, George L.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.05.1994; University of Chicago Press
• Subjects: Adult; Aged; Aged, 80 and over; Bacteremia - immunology; Bacterial diseases; Bacterial sepsis; Biological and medical sciences; Calcimycin - pharmacology; Endotoxins - pharmacology; Female; Gram-Negative Bacterial Infections - immunology; Human bacterial diseases; Humans; Hydroxyeicosatetraenoic Acids - biosynthesis; Infectious diseases; Leukotriene B4 - biosynthesis; Male; Medical sciences; Middle Aged; Neutrophils - metabolism; Human; Leukocyte; Pathogenesis; In vitro; Bacteremia; Blood; HETE; Infection; Calcium ion; Leukotriene B4; Production; Bacteriosis; Microorganism culture; Sensitization; Ionophore; Neutrophil; Gram negative bacteria
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/169.5.1151

The hypothesis was tested that polymorphonuclear leukocytes (PMNL) from patients with gram-negative bacteremia are primed to produce leukotriene B4 (LTB4) or 5-hydroxyeicosatetraenoic acid (5-HETE), in response to concentrations of calcium ionophore A23187, which are substimulatory for control PMNL. PMNL from 11 bacteremic patients and 8 healthy subjects (11 samples) produced similar quantities of LTB4, ω-oxidation products of LTB4 , and 5-HETE after incubation with 0.3 and 0.5 µM A23187 for 5 min. At the detection threshold of 0.3 µM A23187, LTB4 was present in PMNL preparations from 9 of 11 patients and 7 of 11 control samples and 5-HETE from the same 9 patients and from 6 controls. There was no correlation between LTB4 or 5-HETE and plasma levels of endotoxin. In this group of patients, priming of PMNL by gram-negative bacteremia did not lead to enhanced production of LTB4 , its ω-oxidation products, or 5-HETE when PMNL were challenged with low concentrations of A23187.