Characterization of the simultaneous degradation of pyrene and removal of Cr(VI) by a bacteria consortium YH

• Published in: The Science of the total environment Vol. 853; p. 158388
• Main Authors: Su, Yuhua, Sun, Shuo, Liu, Qiyou, Zhao, Chaocheng, Li, Lin, Chen, Shuiquan, Chen, Hongxu, Wang, Yaru, Tang, Fang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 20.12.2022
• Subjects: Bacteria consortium; Bioremediation; Combined pollution; Genome; Metabolic pathway; Bacteria consortium; Metabolic pathway; Combined pollution; Bioremediation; Genome
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.158388

Microorganisms that can simultaneously remediate organic pollutants and heavy metal contamination are great significance in bioremediation. Nevertheless, reports of such microorganisms are still scarce. Here, Pseudomonas sp. YH-1 and Rhodococcus sp. YH-3 were isolated and identified, and they showed greater tolerance to hexavalent (VI) (750 and 800 mg·L−1). The constructed bacteria consortium YH (YH-1:YH-3 = 1:1) could simultaneously degrade 41.69% of pyrene (50 mg·L−1) and remove 76.67% of Cr(VI) (30 mg·L−1) within 5 days. The potential mechanism of Cr(VI) tolerance of YH was further explored by genomic and microscopic analysis. The results showed that YH responded to Cr(VI) stress mainly through efflux of Cr(VI) by chrA and copZ, chromate reduction, DNA-repaired proteases reduces ROS damage, and biosorption by carboxyl, hydroxyl, amino functional groups. Strains YH-1 and YH-3 also contained a variety of genes associated with resistance to other heavy metals, such as cadmium (czcBD), mercury (merAPTR), manganese (mntABC) and copper (copAC, cusABRF and pcoBD). Based on GC–MS and genomic analysis, pyrene was degraded via salicylic acid and phthalic acid pathways. Moreover, a great number of genes related to aromatic hydrocarbon catabolism were identified in the genomes of YH-1 and YH-3. These results confirmed the potential application of the bacteria consortium YH in the bioremediation of water and soil co-contaminated with PAHs-heavy metals. [Display omitted] •A bacteria consortium YH was constructed for pyrene‑chromium bioremediation.•YH was effective in degrading pyrene and removing chromium simultaneously.•The presence of a large number of functional genes for tolerance and removal of pyrene and chromium in YH.