Liposome biodistribution mapping with in vivo X-ray fluorescence imaging

Lipid-based nanoparticles are organic nanostructures constituted of phospholipids and cholesterol, displaying high biocompatibility. They have been demonstrated as effective nanocarriers for drug delivery and targeting. Mapping liposome distribution is crucial as it enables a precise understanding o...

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Bibliographic Details
Published inNanoscale Vol. 16; no. 37; pp. 17404 - 17411
Main Authors Saladino, Giovanni Marco, Chao, Po-Han, Brodin, Bertha, Li, Shyh-Dar, Hertz, Hans Martin
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 26.09.2024
Subjects
Animals
Biocompatibility
Biodistribution
Cell Line, Tumor
Computed tomography
Drug carriers
Efficiency
Encapsulation
Female
Humans
In vivo methods and tests
Lipids
Liposomes
Liposomes - chemistry
Mapping
Medical imaging
Mice
Optical Imaging
Optical properties
Phospholipids
Ruthenium
Ruthenium - chemistry
Tissue Distribution
X ray fluorescence analysis
X ray imagery
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Summary:Lipid-based nanoparticles are organic nanostructures constituted of phospholipids and cholesterol, displaying high biocompatibility. They have been demonstrated as effective nanocarriers for drug delivery and targeting. Mapping liposome distribution is crucial as it enables a precise understanding of delivery kinetics, tissue targeting efficiency, and potential off-target effects. Recently, ruthenium-encapsulated liposomes have shown potential for targeted drug delivery, photodynamic therapy, and optical fluorescence imaging. In the present work, we design Ru(bpy) -encapsulated liposomes (Ru-Lipo) empowering optical and X-ray fluorescence (XRF) properties for dual mode imaging and demonstrate the passivation role of liposomes over the free Ru(bpy) compound. We employ whole-body XRF imaging to map the biodistribution of Ru-Lipo in mice, enabling tumor detection and longitudinal studies with elemental specificity and resolution down to the sub-millimeter scale. Quantitative XRF computed tomography on extracted organs permits targeting efficiency evaluations. These findings highlight the promising role of XRF imaging in pharmacokinetic studies and theranostic applications for the rapid optimization of drug delivery and assessment of targeting efficiency.
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ISSN:2040-3364
2040-3372
2040-3372
DOI:10.1039/d4nr02793k