Cortical high-frequency oscillations (≈ 110 Hz) in cats are state-dependent and enhanced by a subanesthetic dose of ketamine

• Published in: Behavioural brain research Vol. 476; p. 115231
• Main Authors: Castro-Zaballa, Santiago, González, Joaquín, Cavelli, Matías, Mateos, Diego, Pascovich, Claudia, Tort, Adriano, Hunt, Mark Jeremy, Torterolo, Pablo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.01.2025
• Subjects: Anesthesia; Anesthetics, Dissociative - administration & dosage; Anesthetics, Dissociative - pharmacology; Animals; Brain Waves - drug effects; Brain Waves - physiology; Cats; Cerebral Cortex - drug effects; Cerebral Cortex - physiology; Cognition; Electroencephalography - drug effects; Excitatory Amino Acid Antagonists - administration & dosage; Excitatory Amino Acid Antagonists - pharmacology; Female; Gamma; HFO; Ketamine - administration & dosage; Ketamine - pharmacology; Male; Polysomnography; Psychedelic; REM; Sleep - drug effects; Sleep - physiology; Wakefulness - drug effects; Wakefulness - physiology; HFO; Gamma; Anesthesia; Cognition; REM; Psychedelic
• ISSN: 0166-4328; 1872-7549; 1872-7549
• DOI: 10.1016/j.bbr.2024.115231

Ketamine is an NMDA receptor antagonist that has antidepressant and anesthetic properties. At subanesthetic doses, ketamine induces transient psychosis in humans, and is used to model psychosis in experimental animals. In rodents, subanesthetic doses of ketamine increase the power of high-frequency oscillations (HFO, > 100 Hz) in the electroencephalogram (EEG), a frequency band linked to cognitive functions. However, to date, the effects of ketamine in carnivores and primates have been poorly investigated. Here, we examined in the cat, cortical HFO during wakefulness, sleep, and after administering a sub-anesthetic dose of ketamine. Four cats were prepared with cortical electrodes for chronic polysomnographic recordings in head-restrained conditions. The cortical HFO power, connectivity, direction of the information flow using Granger Causality (GC) analysis, their relationships with respiratory activity, and the effect of auditory stimulation were analyzed. During wakefulness, but not during sleep, we found that HFO were coupled with the inspiratory phase of the respiration. After ketamine administration, HFO power was enhanced and remained associated with the inspiratory phase. GC analysis suggests that ketamine-enhanced HFO originate from the olfactory bulb (OB) and stream towards the prefrontal cortex (Pf). Accordingly, occluding the nostrils significantly reduced the power of the ketamine-enhanced HFO in both the OB and Pf. Finally, auditory stimulation did not affect HFO. In conclusion, the HFO are associated with respiration during wakefulness, but not during sleep. The enhancement of this rhythm by ketamine may disrupt cortical information processing, which could contribute to some of the neuropsychiatric effects associated with ketamine. [Display omitted] •High-frequency oscillations (HFO) are modulated by respiration during wakefulness.•Subanesthetic doses of ketamine increase the power of HFO.•Under ketamine, HFO are coupled with the inspiratory phase of respiration.•Ketamine-induced HFO originate in the olfactory bulb and stream to the neocortex.•HFO were not affected by auditory stimulation.