Vascular Endothelial Growth Factor165-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 26; no. 5; pp. 621 - 624
• Main Author: 王彦君 孔维佳 乐建新 孙大为 李伟 姚琪 孙宇 董继华
• Format: Journal Article
• Language: English
• Published: China Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China%Laboratory of Virology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2006
• Subjects: Cell Line, Tumor; Enzyme Activation; Humans; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Nasopharyngeal Neoplasms - metabolism; Nasopharyngeal Neoplasms - pathology; Neoplasm Invasiveness; Neoplasm Metastasis; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transfection; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; 内皮生长因子; 鼻咽癌; nasopharyngeal carcinoma cells; matrix metalloproteinase-2; vascular endothelial growth factor; gene transfection
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-006-0538-z

The effect of vascular endothelial growth factor （VEGF） overexpression on matrix metalloproteinase-2 （MMP-2） in nasopharyngeal carcinoma （NPC） cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF 165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion.