Immunomagnetic Indirect Positive Sorting of Precartilaginous Stem Cells from Neonatal Rat

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 26; no. 6; pp. 723 - 724
• Main Author: 程浩 陈安民 游洪波
• Format: Journal Article
• Language: English
• Published: China Department of Orthopedics, Tongji Hospital, Tongji Medical College of Huangzhong University of Scienc e & Technology 2006
• Subjects: Animals; Animals, Newborn; Cartilage - cytology; Cells, Cultured; Immunomagnetic Separation - methods; Rats; Rats, Sprague-Dawley; Stem Cells - cytology; 干细胞; 新生儿; 软骨膜; magnetic beads; precartilaginous stem cell (PCSC); anti-fibroblast growth factor receptor-3 (anti-FGFR-3)
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-006-0626-0

To investigate the technique of sorting high-purity precartilaginous stem cells from rat＇s perichondrium, neonatal rat＇s perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 （anti-FGFR-3）, and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody, Purity of the sorted neural stem cells was found to be 93.0 %-99.0 %, with living cells amounting to 80 % -85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.