Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD 260 ). Here, we describe a method for direct dete...

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Bibliographic Details
Published inPeerJ analytical chemistry Vol. 4; p. e20
Main Authors Chillar, Komal, Yin, Yipeng, Eriyagama, Adikari M. Dhananjani N., Fang, Shiyue
Format Journal Article
LanguageEnglish
Published PeerJ Inc 01.01.2022
Subjects
HPLC
Oligonucleotide
Optical density
Quantification
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Summary:Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD 260 ). Here, we describe a method for direct determination of OD 260 of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.
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Author ContributionsKomal Chillar performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.Yipeng Yin performed the experiments, authored or reviewed drafts of the article, and approved the final draft.Adikari M. Dhananjani N Eriyagama performed the experiments, authored or reviewed drafts of the article, and approved the final draft.Shiyue Fang conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.
ISSN:2691-6630
2691-6630
DOI:10.7717/peerj-achem.20