Large genomic deletion linked to field-evolved resistance to Cry1F corn in fall armyworm (Spodoptera frugiperda) from Florida
Abstract The fall armyworm ( Spodoptera frugiperda ) is a highly polyphagous lepidopteran pest of relevant food and fiber staple crops. In the Americas, transgenic corn and cotton producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) have controlled and reduced the damage ca...
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Published in | Scientific reports Vol. 12; no. 1; pp. 1 - 9 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group
09.08.2022
Nature Publishing Group UK Nature Portfolio |
Subjects | |
Online Access | Get full text |
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Summary: | Abstract
The fall armyworm (
Spodoptera frugiperda
) is a highly polyphagous lepidopteran pest of relevant food and fiber staple crops. In the Americas, transgenic corn and cotton producing insecticidal proteins from the bacterium
Bacillus thuringiensis
(Bt) have controlled and reduced the damage caused by
S. frugiperda
. However, cases of field-evolved
S. frugiperda
resistance to Bt corn producing the Cry1F insecticidal protein have been documented in North and South America. When characterized, field resistance to Cry1F is linked to insertions and mutations resulting in a modified or truncated ABC transporter subfamily C2 (
SfABCC2
) protein that serves as Cry1F receptor in susceptible
S. frugiperda
. In this work, we present detection of a large genomic deletion (~ 8 kb) affecting the
SfABCC2
and an ABC transporter gene subfamily 3 –like gene (
SfABCC3
) as linked to resistance to Cry1F corn in a
S
.
frugiperda
strain from Florida (FL39). Monitoring for this genomic deletion using a discriminatory PCR reaction in field-collected
S. frugiperda
moths detected individuals carrying this allele in Florida, but not in surrounding states. This is the first report of a large genomic deletion being involved in resistance to a Bt insecticidal protein. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-022-17603-3 |