Serum Proteomic Predictors of Calcification of Solitary Cysticercus Granuloma

• Published in: The American journal of tropical medicine and hygiene Vol. 111; no. 5; pp. 1015 - 1019
• Main Authors: Pamela, Betcy Evangeline, Thamizhmaran, Subashini, Jackson, Elias, Vasudevan, Prabhakaran, Moorthy, Ranjith K., Manoj, Josephin, Thanigachalam, Anupriya, Drevets, Douglas A., Carabin, Hélène, Oommen, Anna, Rajshekhar, Vedantam
• Format: Journal Article
• Language: English
• Published: United States Institute of Tropical Medicine 06.11.2024
• Subjects: Adolescent; Adult; Animals; Biomarkers - blood; Calcification; Calcinosis - blood; Child; Cysticercosis - blood; Cysticercus; Extracellular Matrix Proteins - blood; Female; Granuloma - blood; Granulomas; Humans; Immediate-Early Proteins - blood; Male; Proteomics; Young Adult
• ISSN: 0002-9637; 1476-1645; 1476-1645
• DOI: 10.4269/ajtmh.24-0325

Solitary cysticercus granulomas (SCG), prevalent among Indian patients with new-onset seizures, either resolve completely or transform into calcified granulomas. This study aimed to identify serum proteins through liquid chromatography–tandem mass spectrometry that could predict calcification of SCGs in 20 patients with SCG with at least 6-months of follow-up. At a median follow-up of 14 months, the SCG had calcified ( n = 5), resolved ( n = 11), or persisted ( n = 4). Two serum proteins, ECM1 and MST1, were present in significantly higher serum concentrations, and AZGP1 in significantly lower concentration in subjects with calcifying SCGs than in those with lesions that resolved or persisted. On multivariate analysis, only ECM1 (odds ratio: 1.7, 95% CI: 0–2.8) and MST1 (odds ratio: 3.3, 95% CI: 0–4.1) were independent predictors of calcification of SCG. Combining elevated levels of serum ECM1 and MST1 had a sensitivity of 100% and specificity of 100% in differentiating granulomas that calcified from those that resolved/persisted. Increased expression of serum ECM1 and MST1 in patients with SCG might predict calcification.