High-Performance Graphene-Modified Sensing Chip for SARS-CoV-2 Detection

• Published in: Journal of visualized experiments no. 195
• Main Authors: Sharma, Parshant Kumar, Mostafavi, Ebrahim, Kim, Nam-Young, Webster, Thomas J, Kaushik, Ajeet
• Format: Journal Article
• Language: English
• Published: United States 05.05.2023
• Subjects: Antibodies, Viral; Biosensing Techniques - methods; COVID-19 - diagnosis; Graphite; Humans; SARS-CoV-2; Viruses
• ISSN: 1940-087X
• DOI: 10.3791/64730

This sensing prototype model involves the development of a reusable, twofold graphene oxide (GrO)-glazed double inter-digitated capacitive (DIDC) detecting chip for detecting severe acute respiratory syndrome coronavirus 2 virus (SARS-CoV-2) specifically and rapidly. The fabricated DIDC comprises a Ti/Pt-containing glass substrate glazed with graphene oxide (GrO), which is further chemically modified with EDC-NHS to immobilize antibodies (Abs) hostile to SARS-CoV-2 based on the spike (S1) protein of the virus. The results of insightful investigations showed that GrO gave an ideal engineered surface for Ab immobilization and enhanced the capacitance to allow higher sensitivity and low sensing limits. These tunable elements helped accomplish a wide sensing range (1.0 mg/mL to 1.0 fg/mL), a minimum sensing limit of 1 fg/mL, high responsiveness and good linearity of 18.56 nF/g, and a fast reaction time of 3 s. Besides, in terms of developing financially viable point-of-care (POC) testing frameworks, the reusability of the GrO-DIDC biochip in this study is good. Significantly, the biochip is specific against blood-borne antigens and is stable for up to 10 days at 5 °C. Due to its compactness, this scaled-down biosensor has the potential for POC diagnostics of COVID-19 infection. This system can also detect other severe viral diseases, although an approval step utilizing other virus examples is under development.