The effect of L-carnosine on erythrocyte deformability and aggregation according to the cell age in young and aged rats

• Published in: Acta medica Lékarskí fakulty Univerzity Karlovy v Hradci Králove Vol. 56; no. 1; pp. 23 - 28
• Main Authors: Erken, Gülten, Bor-Kucukatay, Melek, KilicToprak, Emine, Akdag, Beyza, Kucukatay, Vural
• Format: Journal Article
• Language: English
• Published: Czech Republic Karolinum Press 2013
• Subjects: Age Factors; Aged rat; Animals; Carnosine - pharmacology; Cellular Senescence - physiology; Density separation; Erythrocyte Aggregation - drug effects; Erythrocyte deformability; Erythrocyte Deformability - drug effects; L-carnosine; Male; Oxidative Stress - physiology; Rats; Rats, Sprague-Dawley; Total antioxidant status; Total oxidant status
• ISSN: 1211-4286; 1805-9694
• DOI: 10.14712/18059694.2014.34

This study aimed to investigate alterations in hemorheology induced by L-carnosine, an anti- oxidant dipeptide, and to determine their relationship to oxidative stress in density-separated erythrocytes of aged and young rats. 28 male Sprague Dawley rats were divided into 4 groups as aged (Aca), young (Yca) L-carnosine groups (250 mg/kg L-carnosine, i.p.) and aged (As), young (Ys) control groups (saline, i.p.). Density separation was further performed to these groups in order to separate erythrocytes according to their age. Blood samples were used for the determination of erythrocyte deformability, aggregation; and oxidative stress parameters. Erythrocyte deformability of Yca group measured at 0.53 Pa was lower than Aca group. Similarly, deformability of least-dense (young) erythrocytes of Yca group was decreased compared to least-dense erythrocytes of Aca groups. Total antioxidant capacity (TAC) of Aca group was higher and oxidative stress index (OSI) lower than As group. Although L-carnosine resulted in an enhancement in TAC of aged rats, this favorable effect was not observed in erythrocyte deformability and aggregation in the dose applied in this study.