Comparative Chemical Analysis and Bioactive Properties of Aqueous and Glucan-Rich Extracts of Three Widely Appreciated Mushrooms: Agaricus bisporus (J.E.Lange) Imbach, Laetiporus sulphureus (Bull.) Murill and Agrocybe aegerita (V. Brig.) Vizzini

• Published in: Pharmaceuticals (Basel, Switzerland) Vol. 17; no. 9; p. 1153
• Main Authors: Petrović, Jovana, Glamočlija, Jasmina, Milinčić, Danijel D, Doroški, Ana, Lević, Steva, Stanojević, Slađana P, Kostić, Aleksandar Ž, Minić, Dušanka A Popović, Vidović, Bojana B, Plećić, Ana, Nedović, Viktor A, Pešić, Mirjana B, Stojković, Dejan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.08.2024; MDPI
• Subjects: anti-inflammatory agents; antimicrobial agents; antioxidant agents; Antioxidants; ATR-FTIR; chemical characterization; Dietary minerals; Fatty acids; Food products; Ingredients; mushroom extracts; Mushrooms; Polypeptides; Proteins; antioxidant agents; antimicrobial agents; ATR-FTIR; mushroom extracts; chemical characterization; anti-inflammatory agents; wound-healing properties
• ISSN: 1424-8247; 1424-8247
• DOI: 10.3390/ph17091153

Herein we describe the antioxidant, antimicrobial, antibiofilm, anti-inflammatory and wound-healing potential of aqueous and polysaccharide extracts from three widely appreciated mushrooms: , and . Moreover, we present their detailed phenolic, polysaccharide and protein profiles and ATR-FTIR spectra. The study found that polysaccharide extracts (PEs) from mushrooms had higher total and -glucan levels than aqueous extracts (AEs), with showing the highest content. had a higher total protein content, and AE had the highest phenolic content. Our results indicate that all the tested extracts have high potential regarding their bioactive properties, with being the most promising one. Namely, the antibacterial activity assay showed that the development of the skin-infection-causing agent, , was inhibited with a minimal inhibitory concentration of 4.00 mg/mL and minimal bactericidal concentration of 8.00 mg/mL, while the results regarding wound healing showed that, over the course of 24 h, the extract actively promoted wound closure in the HaCaT keratinocyte cell line model. The anti-inflammatory activity results clearly showed that when we used as an inflammation-inducing agent and the aqueous extract in treatment, IL-6 levels reduced to the level of 4.56 pg/mL. The obtained data suggest that the tested mushroom extracts may serve as a source of bioactive compounds, with potential applications in the cosmeceutical, pharmaceutical and food industries. Furthermore, potential skin preparations carefully crafted with mushroom extract may help restore the skin's barrier function, decrease the probability of staph infections and minimize skin irritation.