Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells

• Published in: Obstetrics & gynecology science Vol. 57; no. 6; pp. 501 - 506
• Main Authors: Lee, Hyun Gyo, Lee, Ji Min, Shin, So Jin, Kwon, Sang Hoon, Lee, Gi Su, Song, Chang Ho, Choi, Eun Som, Cha, Soon Do, Cho, Chi Heum
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society of Obstetrics and Gynecology; Korean Society of Contraception and Reproductive Health; Korean Society of Gynecologic Endocrinology; Korean Society of Gynecologic Endoscopy and Minimal Invasive Surgery; Korean Society of Maternal Fetal Medicine; Korean Society of Ultrasound in Obstetrics and Gynecology; Korean Urogynecologic Society 01.11.2014; 대한산부인과학회
• Subjects: Original; 산부인과학; Leiomyoma; Extrinsic; Salinomycin; Intrinsic; Apoptosis
• ISSN: 2287-8572; 2287-8580
• DOI: 10.5468/ogs.2014.57.6.501

The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot. The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed. These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.