Molecular analysis of two genes between let-653 and let-56 in the unc-22(4) region of Caenorhabditis elegans

• Published in: Molecular & general genetics Vol. 236; no. 2-3; p. 289
• Main Authors: Marra, M.A. (Simon Fraser Univ., Burnaby (Canada). Dept. of Biological Sciences. Inst. for Molecular Biology and Biochemistry), Prasad, S.S, Baillie, D.L
• Format: Journal Article
• Language: English
• Published: Germany 1993
• Subjects: ADN; Amino Acid Sequence; Animals; Base Sequence; CAENORHABDITIS ELEGANS; Caenorhabditis elegans - genetics; Carrier Proteins - genetics; Chromosome Mapping; Cloning, Molecular; Cosmids; Deoxyribonucleases, Type II Site-Specific - metabolism; DNA Probes; DOPA; Dopa decarboxylase; Dopa Decarboxylase - genetics; GENE; Gene Library; GENES; Genes, Helminth - genetics; GENOMAS; GENOME; Genomic organization; LIASAS; LYASE; Molecular Sequence Data; Na(+)/H(+) antiporter; Open Reading Frames; Polymerase Chain Reaction; Protein Conformation; SECUENCIA NUCLEICA; Sequence Analysis, DNA; SEQUENCE NUCLEIQUE; Sodium-Hydrogen Exchangers; Solubility; Transcription, Genetic
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/BF00277125

A previous study of genomic organization described the identification of nine potential coding regions in 150 kb of genomic DNA from the unc-22(4) region of Caenorhabditis elegans. In this study, we focus on the genomic organization of a small interval of 0.1 map unit bordered on the right by unc-22 and on the left by the left-hand breakpoints of the deficiencies sDf9, sDf19 and sDf65. This small interval at present contains a single mutagenically defined locus, the essential gene let-56. The cosmid C11F2 has previously been used to rescue let-56. Therefore, at least some of C11F2 must reside in the interval. In this paper two coding elements that reside on C11F2 are characterized. Analysis of nucleotide sequence data obtained from cDNAs and cosmid subclones revealed that one of the coding elements closely resembles aromatic amino acid decarboxylases from several species. The other of these coding elements was found to closely resemble a human growth factor activatable Na(+)/H(+) antiporter. Pairs of oligonucleotide primers, predicted from both coding elements, have been used in PCR experiments to position these coding elements between the left breakpoint of sDf19 and the left breakpoint of sDf65, between the essential genes let-653 and let-56.