Development and validation of an LC-MS/MS method for the quantification of fascin proteins in human serum

Fascin is an actin-bundling protein that has been linked to tumor cell migration, invasion, metastasis, disease progression and mortality, thus serving as a novel cancer biomarker. Bioanalytical methods to measure fascin in biological matrices are sparsely reported, while accurate quantitation of fa...

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Bibliographic Details
Published inBioanalysis Vol. 14; no. 16; pp. 1095 - 1109
Main Authors Li, Ke, Zhang, Zhiling, Wang, Kai, Huang, Xin-Yun, Lin, Zhongping (John)
Format Journal Article
LanguageEnglish
Published Newlands Press Ltd 01.08.2022
Subjects
cancer protein biomarker
fascin
LC-MS/MS
method validation
pellet digestion
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Summary:Fascin is an actin-bundling protein that has been linked to tumor cell migration, invasion, metastasis, disease progression and mortality, thus serving as a novel cancer biomarker. Bioanalytical methods to measure fascin in biological matrices are sparsely reported, while accurate quantitation of fascin levels may lend support for fascin as a promising therapeutic target. An LC-MS/MS-based method involving protein precipitation, enzymatic digestion and solid phase extraction was developed and validated for the quantitation of fascin in human serum. Linearity over a calibration range of 5–500 ng/ml with a LLOQ of 5 ng/ml, great accuracy and precision, excellent parallelism as well as high extraction recovery were achieved. This method provides a valuable tool for anticancer drug development and cancer treatment.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2022-0121