Small-angle X ray scattering studies on whole bacillus subtilis

Whole and broken Bacillus subtilis bacteria were studied by shall-angle X ray scattering in the region from 3–18 milliradians, to investigate the condition of ribosomes in whole bacteria. The scattering curves for the whole bacteria were of lower intensity than those for the broken bacteria, but mos...

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Published inJournal of ultrastructure research Vol. 52; no. 3; pp. 300 - 308
Main Authors Gee, Merrill K., Parker, Dennis L., Ford, Larry C., Dibble, William E.
Format Journal Article
LanguageEnglish
Published United States Elsevier B.V 01.01.1975
Subjects
Bacillus subtilis - ultrastructure
Ribosomes - ultrastructure
Scattering, Radiation
X-Rays
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Summary:Whole and broken Bacillus subtilis bacteria were studied by shall-angle X ray scattering in the region from 3–18 milliradians, to investigate the condition of ribosomes in whole bacteria. The scattering curves for the whole bacteria were of lower intensity than those for the broken bacteria, but most of the difference appears to be due to changes of interparticle interference of protein and ribosomes when the cell contents are diluted upon rupture of the bacteria. Scattering, studies on solutions of ribosomes and bovine plasma albumin mixed together showed similar behavior of the scattering patterns upon dilution.
ISSN:0022-5320
1878-2345
DOI:10.1016/S0022-5320(75)80070-0