Plasma membrane Ca2+ ATPase isoform 1 down-regulated in human oral cancer

• Published in: Oncology reports Vol. 15; no. 1; pp. 49 - 55
• Main Authors: SAITO, Kengo, UZAWA, Katsuhiro, ENDO, Yosuke, KATO, Yoshikuni, NAKASHIMA, Dai, OGAWARA, Katsunori, SHIBA, Masashi, BUKAWA, Hiroki, YOKOE, Hidetaka, TANZAWA, Hideki
• Format: Journal Article
• Language: English
• Published: Athens S.n. 2006
• Subjects: Biological and medical sciences; Calcium-Transporting ATPases - analysis; Calcium-Transporting ATPases - genetics; Cation Transport Proteins - analysis; Cation Transport Proteins - genetics; DNA Methylation; DNA Mutational Analysis; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Medical sciences; Mouth Neoplasms - chemistry; Mouth Neoplasms - genetics; Otorhinolaryngology. Stomatology; Plasma Membrane Calcium-Transporting ATPases; RNA, Messenger - analysis; RNA, Messenger - metabolism; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; Immunohistochemistry; Molecular form; RNA-directed DNA polymerase; Western blot analysis; Calcium; Oral cancer; plasma membrane Ca2+ ATPase; Inorganic element; real-time quantitative RT-PCR; Cancerology; Immunoblotting assay; DNA methylation; Reverse transcription polymerase chain reaction; Quantitative analysis; Tumor suppressor gene; Human; Enzyme; Stomatology; Transferases; Adenosinetriphosphatase; Malignant tumor; Oral squamous cell carcinoma; Real time; Oral cavity; Anatomic pathology; Nucleotidyltransferases; DNA; Plasma membrane; Hydrolases; Oral cavity disease; Molecular biology; Methylation
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or.15.1.49

The plasma membrane Ca(2+) ATPase (PMCA) is an essential regulator of free intracellular calcium. Recent studies have reported aberrant expression of the PMCA1 gene, a member of the PMCA family, in several cancer cell types. To elucidate the contribution of PMCA1 to oral carcinogenesis, we analyzed genetic and epigenetic changes and mRNA and protein expression in primary oral squamous cell carcinomas (OSCCs), oral premalignant lesions (OPLs), and OSCC-derived cell lines. The PMCA1 gene was epigenetically inactivated, but not mutated in the eight OSCC-derived cell lines tested. In clinical samples, frequent down-regulation of PMCA1 protein expression was found not only in primary OSCCs (43%), but also in OPLs (40%). Real-time quantitative reverse transcriptase-polymerase chain reaction data were consistent with the protein expression status. These results suggest that inactivation of the PMCA1 gene is a frequent and early event during oral carcinogenesis, and gene expression may be regulated by an epigenetic mechanism.