Evaluation of PCR mediated DNA amplification in non‐invasive biological specimens for subclinical detection of Mycobacterium leprae

DNA from Mycobacterium leprae, present in non‐invasive clinical samples from leprosy patients, such as nasal secretion and hair bulbs, was submitted to amplification by the polymerase chain reaction using a M. leprae‐specific repetitive sequence as a target. After optimization of sample processing a...

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Published inFEMS immunology and medical microbiology Vol. 11; no. 2; pp. 113 - 120
Main Authors Santos, Adalberto R., Filho, Jose T.Goes, Nery, Jose A.C., Duppre, Nadia C., Gallo, Maria E.N., Suffys, Philip N., Degrave, Wim M.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.04.1995
Blackwell
Subjects
Adolescent
Adult
Aged
Bacterial diseases
Biological and medical sciences
Child
Clinical sample
DNA, Bacterial - analysis
Evaluation Studies as Topic
Female
Hair - microbiology
Human bacterial diseases
Humans
Infectious diseases
Leprosy
Leprosy - diagnosis
Leprosy - microbiology
Male
Medical sciences
Middle Aged
Mycobacterium leprae
Mycobacterium leprae - genetics
Mycobacterium leprae - isolation & purification
Nasal Mucosa - microbiology
Nasal Mucosa - secretion
Polymerase Chain Reaction
Tropical bacterial diseases
Performance evaluation
Skin disease
Mycobacterium leprae
Leprosy
Mycobacterial infection
Infection
Polymerase chain reaction
Mycobacteriales
DNA
Bacteriosis
Mycobacteriaceae
Bacteria
Actinomycetes
Diagnosis
Detection
Clinical isolate
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Summary:DNA from Mycobacterium leprae, present in non‐invasive clinical samples from leprosy patients, such as nasal secretion and hair bulbs, was submitted to amplification by the polymerase chain reaction using a M. leprae‐specific repetitive sequence as a target. After optimization of sample processing and of the PCR conditions, we were able to detect DNA from M. leprae in both types of clinical samples, even from paucibacillary leprosy patients. The use of hair bulbs and nasal secretion as clinical samples for screening of household contacts and for the evaluation of a risk population, or for the follow‐up of patients under chemotherapy, and monitoring of bacterial load is discussed.
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ISSN:0928-8244
1574-695X
DOI:10.1111/j.1574-695X.1995.tb00097.x