Cross-linked albumin as supporting matrix in ultrathin cryo microtomy

A method is described by which tissue blocks and cell cultures can be rapidly encapsulated for cryoultramicrotomy. Bovine serum albumin, insolubilized with glutaraldehyde, has been found to be a good embedding medium for biological purposes. Ultrathin frozen sections with large fields that are suffi...

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Bibliographic Details
Published inJournal of ultrastructure research Vol. 41; no. 3; pp. 385 - 394
Main Authors Kuhlmann, W.D., Viron, A.
Format Journal Article
LanguageEnglish
Published United States Elsevier B.V 01.11.1972
Subjects
Aldehydes
Animals
Cells, Cultured
Glutarates
Kidney - cytology
Liver - cytology
Methods
Microscopy, Electron
Microtomy
Optic Nerve - cytology
Rats
Serum Albumin, Bovine
Stomach - cytology
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Summary:A method is described by which tissue blocks and cell cultures can be rapidly encapsulated for cryoultramicrotomy. Bovine serum albumin, insolubilized with glutaraldehyde, has been found to be a good embedding medium for biological purposes. Ultrathin frozen sections with large fields that are sufficiently uniform for low and high magnification micrographs can be readily obtained. The fine structure of cells and at least some of their enzymatic and chemical properties can be preserved by the described procedures.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-5320
1878-2345
DOI:10.1016/S0022-5320(72)90078-0