Separation of serotonin metabolites from urine on cellulose columns

A procedure for the separation of serotonin metabolites by cellulose column chromatography is described: 24-hr urine from 3–6 rats treated with labeled serotonin was freed from salts, urea, and 5-hydroxyindole-3-acetic acid and passed through a cellulose column. A partial resolution of metabolites i...

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Published inAnalytical biochemistry Vol. 7; no. 3; pp. 297 - 303
Main Authors ISKRIC, S, KEGLEVIC, D
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.03.1964
Subjects
Carbon Isotopes
Cellulose
Chromatography
Hydroxyindoleacetic Acid
Indoleacetic Acids
Indoles
Old Medline
Rats
Serotonin
Urine
CARBON ISOTOPES
HYDROXYINDOLEACETIC ACID
EXPERIMENTAL LAB STUDY
INDOLEACETIC ACID
URINE
RATS
SEROTONIN
CHROMATOGRAPHY
INDOLES
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Summary:A procedure for the separation of serotonin metabolites by cellulose column chromatography is described: 24-hr urine from 3–6 rats treated with labeled serotonin was freed from salts, urea, and 5-hydroxyindole-3-acetic acid and passed through a cellulose column. A partial resolution of metabolites in four discrete peaks was achieved. The individual peaks were further subjected to column and paper chromatography, respectively. By this procedure, in addition to 5-hydroxyindole-3-acetic acid, six radiochemically pure serotonin metabolites of indolic structure were obtained.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(64)90134-4