The S1 to S2 and S2 to S3 state transitions in plant photosystem II: relevance to the functional and structural heterogeneity of the water oxidizing complex

• Published in: Photosynthesis research
• Main Authors: Pavlou, Andrea, Styring, Stenbjörn, Mamedov, Fikret
• Format: Journal Article
• Language: English
• Published: 25.04.2024
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-024-01096-4

Abstract In Photosystem II, light-induced water splitting occurs via the S state cycle of the CaMn 4 O 5 -cluster. To understand the role of various possible conformations of the CaMn 4 O 5 -cluster in this process, the temperature dependence of the S 1  → S 2 and S 2  → S 3 state transitions, induced by saturating laser flashes, was studied in spinach photosystem II membrane preparations under different conditions. The S 1  → S 2 transition temperature dependence was shown to be much dependent on the type of the cryoprotectant and presence of 3.5% methanol, resulting in the variation of transition half-inhibition temperature by 50 K. No similar effect was observed for the S 2  → S 3 state transition, for which we also show that both the low spin g = 2.0 multiline and high spin g = 4.1 EPR configurations of the S 2 state advance with similar efficiency to the S 3 state, both showing a transition half-inhibition temperature of 240 K. This was further confirmed by following the appearance of the Split S 3 EPR signal. The results are discussed in relevance to the functional and structural heterogeneity of the water oxidizing complex intermediates in photosystem II.