Semen cryopreservation of Piau swine breed submited to three freezing protocols

The objective of this study was to verify the effect of three protocols of cryopreservation on the sperm viability post-thawing of swine from the Piau breed (Sus scrofa), by means of evaluation assays in vitro and in vivo. Twenty-two ejaculates of 5 adult males of the Piau breed trough the gloved-ha...

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Published inRevista brasileira de zootecnia Vol. 41; no. 4; pp. 914 - 922
Main Authors Barros, Maurício Hoshino da Costa, Shiomi, Hugo Hideki, Amorim, Lincoln da Silva, Guimarães, Simone Eliza Facioni, Lopes, Paulo Sávio, Siqueira, Jeanne Broch, Guimarães, José Domingos
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Portuguese
Published Sociedade Brasileira de Zootecnia 01.04.2012
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Abstract The objective of this study was to verify the effect of three protocols of cryopreservation on the sperm viability post-thawing of swine from the Piau breed (Sus scrofa), by means of evaluation assays in vitro and in vivo. Twenty-two ejaculates of 5 adult males of the Piau breed trough the gloved-hand method. In order to verify the sperm viability of the raw semen and post-thawing, the following traits were evaluated: sperm motility and vigor, hypoosmotic test, supra-vital color test and percentage of normal acrosomes. To freezing, the ejaculates were fractioned and submitted in: method 1, method of freezing recommended by Fürst et al. (2005), modified according to media extender; method 2, recommended by Fürst et al. (2005), modified according to the cooling curve; and method 3, recommended by Ohata et al. (2001). To test the in vivo fertility of the frozen/thawed semen, 23 intra-uterine (post-cervical) inseminations were performed in 14 females of the Piau breed, with semen from method 3. The mean values obtained with the use of methods 1 and 2 and post-thawing were inferior to those obtained with method 3. Four adult sows got pregnant (28.6% fertility rate). The freezing method recommended by Ohata et al. (2001), with balance period of 90 minutes at 22-26 ºC, enables higher results of in vitro sperm viability in comparison with the two other methods. The absence of balance period of method 1 and the cooling curve utilized in protocol 2 affect negatively the parameters of sperm viability evaluated in the present study. In spite of the satisfactory results in vitro for the freezing of semen with the utilization of method 3, it presents low fertility rate (28.6%). Objetivou-se verificar o efeito de três protocolos de criopreservação sobre a viabilidade espermática pósdescongelamento de suínos da raça Piau (Sus scrofa), por meio de testes de avaliação in vitro e in vivo. Foram coletados 22 ejaculados de 5 machos adultos da raça Piau por meio do método da mão enluvada. Para verificar a viabilidade espermática do sêmen in natura e pós-descongelamento, foram avaliados a motilidade e o vigor espermático, o teste hiposmótico, a coloração supra-vital e a porcentagem de acrossomas normais. Para o congelamento, os ejaculados foram fracionados e submetidos em: protocolo 1, método de congelamento preconizado por Fürst et al. (2005), modificado quanto aos meios diluentes; protocolo 2, método preconizado por Fürst et al. (2005), modificado quanto à curva de resfriamento; e protocolo 3, método preconizado por Ohata et al. (2001). Para testar a fertilidade in vivo do sêmen congelado/descongelado, foram realizadas 23 inseminações intrauterinas (pós-cervicais) em 14 fêmeas da raça Piau com o sêmen proveniente do protocolo 3. Os valores médios obtidos com o uso dos protocolos 1 e 2 pós-descongelamento foram inferiores aos obtidos com o protocolo 3. Quatro porcas adultas ficaram gestantes (28,6% de taxa de fertilidade). O protocolo de congelamento preconizado por Ohata et al. (2001), com período de equilíbrio de 90 minutos a 22-26 ºC, permite obter melhores resultados de viabilidade espermática in vitro em comparação aos outros dois protocolos. A ausência de período de equilíbrio do protocolo 1 e a curva de resfriamento utilizada no protocolo 2 afetam negativamente os parâmetros de viabilidade espermática avaliados no presente estudo. Apesar dos resultados satisfatórios in vitro para o congelamento de sêmen com a utilização do protocolo 3, o mesmo apresenta baixa taxa de fertilidade (28,6%).
AbstractList The objective of this study was to verify the effect of three protocols of cryopreservation on the sperm viability post-thawing of swine from the Piau breed (Sus scrofa), by means of evaluation assays in vitro and in vivo. Twenty-two ejaculates of 5 adult males of the Piau breed trough the gloved-hand method. In order to verify the sperm viability of the raw semen and post-thawing, the following traits were evaluated: sperm motility and vigor, hypoosmotic test, supra-vital color test and percentage of normal acrosomes. To freezing, the ejaculates were fractioned and submitted in: method 1, method of freezing recommended by Fürst et al. (2005), modified according to media extender; method 2, recommended by Fürst et al. (2005), modified according to the cooling curve; and method 3, recommended by Ohata et al. (2001). To test the in vivo fertility of the frozen/thawed semen, 23 intra-uterine (post-cervical) inseminations were performed in 14 females of the Piau breed, with semen from method 3. The mean values obtained with the use of methods 1 and 2 and post-thawing were inferior to those obtained with method 3. Four adult sows got pregnant (28.6% fertility rate). The freezing method recommended by Ohata et al. (2001), with balance period of 90 minutes at 22-26 ºC, enables higher results of in vitro sperm viability in comparison with the two other methods. The absence of balance period of method 1 and the cooling curve utilized in protocol 2 affect negatively the parameters of sperm viability evaluated in the present study. In spite of the satisfactory results in vitro for the freezing of semen with the utilization of method 3, it presents low fertility rate (28.6%). Objetivou-se verificar o efeito de três protocolos de criopreservação sobre a viabilidade espermática pósdescongelamento de suínos da raça Piau (Sus scrofa), por meio de testes de avaliação in vitro e in vivo. Foram coletados 22 ejaculados de 5 machos adultos da raça Piau por meio do método da mão enluvada. Para verificar a viabilidade espermática do sêmen in natura e pós-descongelamento, foram avaliados a motilidade e o vigor espermático, o teste hiposmótico, a coloração supra-vital e a porcentagem de acrossomas normais. Para o congelamento, os ejaculados foram fracionados e submetidos em: protocolo 1, método de congelamento preconizado por Fürst et al. (2005), modificado quanto aos meios diluentes; protocolo 2, método preconizado por Fürst et al. (2005), modificado quanto à curva de resfriamento; e protocolo 3, método preconizado por Ohata et al. (2001). Para testar a fertilidade in vivo do sêmen congelado/descongelado, foram realizadas 23 inseminações intrauterinas (pós-cervicais) em 14 fêmeas da raça Piau com o sêmen proveniente do protocolo 3. Os valores médios obtidos com o uso dos protocolos 1 e 2 pós-descongelamento foram inferiores aos obtidos com o protocolo 3. Quatro porcas adultas ficaram gestantes (28,6% de taxa de fertilidade). O protocolo de congelamento preconizado por Ohata et al. (2001), com período de equilíbrio de 90 minutos a 22-26 ºC, permite obter melhores resultados de viabilidade espermática in vitro em comparação aos outros dois protocolos. A ausência de período de equilíbrio do protocolo 1 e a curva de resfriamento utilizada no protocolo 2 afetam negativamente os parâmetros de viabilidade espermática avaliados no presente estudo. Apesar dos resultados satisfatórios in vitro para o congelamento de sêmen com a utilização do protocolo 3, o mesmo apresenta baixa taxa de fertilidade (28,6%).
The objective of this study was to verify the effect of three protocols of cryopreservation on the sperm viability post-thawing of swine from the Piau breed (Sus scrofa), by means of evaluation assays in vitro and in vivo. Twenty-two ejaculates of 5 adult males of the Piau breed trough the gloved-hand method. In order to verify the sperm viability of the raw semen and post-thawing, the following traits were evaluated: sperm motility and vigor, hypoosmotic test, supra-vital color test and percentage of normal acrosomes. To freezing, the ejaculates were fractioned and submitted in: method 1, method of freezing recommended by Fürst et al. (2005), modified according to media extender; method 2, recommended by Fürst et al. (2005), modified according to the cooling curve; and method 3, recommended by Ohata et al. (2001). To test the in vivo fertility of the frozen/thawed semen, 23 intra-uterine (post-cervical) inseminations were performed in 14 females of the Piau breed, with semen from method 3. The mean values obtained with the use of methods 1 and 2 and post-thawing were inferior to those obtained with method 3. Four adult sows got pregnant (28.6% fertility rate). The freezing method recommended by Ohata et al. (2001), with balance period of 90 minutes at 22-26 ºC, enables higher results of in vitro sperm viability in comparison with the two other methods. The absence of balance period of method 1 and the cooling curve utilized in protocol 2 affect negatively the parameters of sperm viability evaluated in the present study. In spite of the satisfactory results in vitro for the freezing of semen with the utilization of method 3, it presents low fertility rate (28.6%).
Author Barros, Maurício Hoshino da Costa
Shiomi, Hugo Hideki
Guimarães, José Domingos
Siqueira, Jeanne Broch
Guimarães, Simone Eliza Facioni
Lopes, Paulo Sávio
Amorim, Lincoln da Silva
AuthorAffiliation Universidade Federal de Viçosa
AuthorAffiliation_xml – name: Universidade Federal de Viçosa
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  givenname: Maurício Hoshino da Costa
  surname: Barros
  fullname: Barros, Maurício Hoshino da Costa
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  givenname: Hugo Hideki
  surname: Shiomi
  fullname: Shiomi, Hugo Hideki
  organization: Universidade Federal de Viçosa
– sequence: 3
  givenname: Lincoln da Silva
  surname: Amorim
  fullname: Amorim, Lincoln da Silva
  organization: Universidade Federal de Viçosa
– sequence: 4
  givenname: Simone Eliza Facioni
  surname: Guimarães
  fullname: Guimarães, Simone Eliza Facioni
  organization: Universidade Federal de Viçosa
– sequence: 5
  givenname: Paulo Sávio
  surname: Lopes
  fullname: Lopes, Paulo Sávio
  organization: Universidade Federal de Viçosa
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  givenname: Jeanne Broch
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  givenname: José Domingos
  surname: Guimarães
  fullname: Guimarães, José Domingos
  organization: Universidade Federal de Viçosa
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Issue 4
Keywords espermatozoides
varrões
spermatozoa
intra-uterine insemination
boars
inseminação intra-uterina
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Portuguese
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Snippet The objective of this study was to verify the effect of three protocols of cryopreservation on the sperm viability post-thawing of swine from the Piau breed...
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SubjectTerms AGRICULTURE, DAIRY & ANIMAL SCIENCE
VETERINARY SCIENCES
Title Semen cryopreservation of Piau swine breed submited to three freezing protocols
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