Structure and serological analysis of the Hafnia alvei 481-L O-specific polysaccharide containing phosphate in the backbone chain

• Published in: Carbohydrate research Vol. 341; no. 18; pp. 2980 - 2985
• Main Authors: Kübler-Kiełb, J., Vinogradov, E., García Fernández, J.M., Szostko, B., Zwiefka, A., Gamian, A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 29.12.2006
• Subjects: Animals; Carbohydrate Sequence; Endotoxin; Enterobacteria; Gas Chromatography-Mass Spectrometry; Hafnia alvei; Hafnia alvei - chemistry; Lipopolysaccharide; LPS; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; O-antigen; Polysaccharide; Polysaccharides, Bacterial - chemistry; Polysaccharides, Bacterial - immunology; Rabbits; Serologic Tests; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; Sugar Phosphates - chemistry; PS; OS; O-PS; O-antigen; Hafnia alvei; Endotoxin; LPS; Enterobacteria; Lipopolysaccharide; 1D and 2D; HSQC; Structure; Polysaccharide
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2006.10.007

The lipopolysaccharide was extracted from cells of Hafnia alvei 481-L bacterial strain and, after mild acid hydrolysis, the O-specific polysaccharide was isolated and characterised. On the basis of chemical analyses and NMR spectroscopic studies of the polysaccharide and oligosaccharides obtained after Smith degradation, or hydrogen fluoride treatment, it was found that the repeating unit of the O-specific polysaccharide is a phosphorylated hexasaccharide: [Display omitted] The biological repeating unit of the H. alvei 481-L O-antigen has galactose phosphate at the nonreducing terminus. Serological tests indicate that this strain represents an individual serotype in the H. alvei genus.