Senescence-specific Alteration of Hydrogen Peroxide Levels in Arabidopsis thaliana and Oilseed Rape Spring Variety Brassica napus L. cv. Mozart[]

• Published in: Journal of integrative plant biology Vol. 54; no. 8; pp. 540 - 554
• Main Authors: Bieker, Stefan, Riester, Lena, Stahl, Mark, Franzaring, Jürgen, Zentgraf, Ulrike
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.08.2012
• Subjects: Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis thaliana; ascorbate Peroxidase; Ascorbate Peroxidases - metabolism; Brassica napus; Brassica napus - physiology; catalase; Catalase - metabolism; E. coli Oxy-RD; elevated CO2; Escherichia coli Proteins - genetics; Genes, Plant; Hydrogen Peroxide - metabolism; intracellular hydrogen peroxide; Repressor Proteins - genetics; senescence phenotype; Transcription Factors - genetics; 抗坏血酸过氧化物酶; 拟南芥; 春性品种; 油菜品种; 甘蓝型油菜; 衰老过程; 过氧化氢酶; 过氧化物酶体
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2012.01147.x

In order to analyze the signaling function of hydrogen peroxide （H202） production in senescence in more detail, we manipulated intracellular H202 levels in Arabidopsis thaliala （L.） Heynh by using the hydrogen- peroxide-sensitive part of the Escherichia coil transcription regulator OxyR, which was directed to the cytoplasm as well as into the peroxisomes. H202 levels were lowered and senescence was delayed in both transgenic lines, but OxyR was found to be more effective in the cytoplasm. To transfer this knowledge to crop plants, we analyzed oilseed rape plants Brassica napus L. cv. Mozart for H20~ and its scavenging enzymes catalase （CAT） and ascorbate peroxidase （APX） during leaf and plant development. H202 levels were found to increase during bolting and flowering time, but no increase could be observed in the very late stages of senescence. With increasing H202 levels, CAT and APX activities declined, so it is likely that similar mechanisms are used in oilseed rape and Arabidopsis to control H202 levels. Under elevated CO2 conditions, oilseed rape senescence was accelerated and coincided with an earlier increase in H202 levels, indicating that H202 may be one of the signals to inducing senescence in a broader range of Brassicaceae.