The expression of murine cutaneous late phase reaction requires both IgE antibodies and CD4 T cells

• Published in: Clinical and experimental allergy Vol. 27; no. 2; pp. 225 - 231
• Main Authors: SAWADA, K, NAGAI, H, BASAKI, Y, YAMAYA, H, IKIZAWA, K, WATANABE, M, KOJIMA, M, MATSUURA, N, KINIWA, M
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell 01.02.1997
• Subjects: Allergic diseases; Animals; Antibodies, Monoclonal - pharmacology; Biological and medical sciences; CD4-Positive T-Lymphocytes - immunology; Ear, External; Hypersensitivity, Immediate - etiology; Hypersensitivity, Immediate - immunology; Immunization, Passive; Immunoglobulin E - physiology; Immunopathology; Interleukin-4 - immunology; Male; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Nude; Ovalbumin - administration & dosage; Ovalbumin - immunology; Skin - immunology; Skin - pathology; Skin allergic diseases. Stinging insect allergies; Vaccination; Immunopathology; Allergy; Ovalbumin; Skin disease; Phase; Pathophysiology; IgE; Rodentia; Exploration; Delayed hypersensitivity; Experimental disease; Vertebrata; Mammalia; Mouse; Animal; T-Lymphocyte
• ISSN: 0954-7894; 1365-2222
• DOI: 10.1046/j.1365-2222.1997.d01-489.x

Exposure of atopic patients to a specific allergen evokes an immediate response which is followed, in many cases, by a late phase reaction (LPR) some hours later. Here we have examined the immunological mechanisms required for the expression of cutaneous LPR in mice. BALB/c mice were immunized by i.p. injection of ovalbumin (OVA) and alum actively or by i.v. injection of anti-OVA IgE monoclonal antibody (mAb) passively. After challenge by intradermal injection of OVA into ears, the changes in ear thickness, the number of eosinophils, and the levels of IL-4 and IFN-gamma protein at the site of antigen challenge were examined. Actively immunized mice developed a biphasic response at the site of OVA injection, while mice passively immunized with IgE anti-OVA mAb displayed a strong early response but no LPR. Cell transfer experiments using BALB/c nu/nu mice revealed that both OVA-specific IgE mAb and OVA-primed CD4 T cells were required to evoke LPR. Moreover, LPR was associated with increased levels of IL-4 production concomitant with reduced IFN-gamma production and was abolished by pretreatment with anti-IL-4 neutralizing mAb. It is suggested that murine cutaneous LPR against OVA is a type 2 inflammatory response in which both IgE antibodies and CD4 T cells play an obligatory role.