Induction of G2 -M phase arrest and apoptosis by α-methylene-γ-butyrolactones in human leukemia cells
Abstract In this study, we investigated the effect of three synthetic α-methylene-γ-butyrolactones (MBL) on viability of 10 human tumor cell lines and found that these lactones were highly cytotoxic against leukemia cells. Studies performed on HL-60 cells indicate that MBL induce G2 -M arrest and ap...
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Published in | Cancer letters Vol. 269; no. 1; pp. 139 - 147 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Ireland
28.09.2008
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Subjects | |
Online Access | Get full text |
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Summary: | Abstract In this study, we investigated the effect of three synthetic α-methylene-γ-butyrolactones (MBL) on viability of 10 human tumor cell lines and found that these lactones were highly cytotoxic against leukemia cells. Studies performed on HL-60 cells indicate that MBL induce G2 -M arrest and apoptosis through a caspase-dependent mechanism. Apoptosis was associated to cytochrome c release, cleavage of procaspases-9 and -3, and hydrolysis of PARP. Intracellular reactive oxygen species (ROS) seem to play a key role since high levels of ROS were produced early (<15 min) and apoptosis was completely abrogated by the free radical scavenger N -acetyl- l -cysteine (NAC). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0304-3835 1872-7980 |
DOI: | 10.1016/j.canlet.2008.04.028 |