Effects of protease-resistant antimicrobial substances produced by lactic acid bacteria on rumen methanogenesis

• Published in: Asian-australasian journal of animal sciences Vol. 23; no. 6; pp. 700 - 707
• Main Authors: Asa, Reina, Tanaka, A, Uehara, A, Shinzato, I, Toride, Y, Usui, N, Hirakawa K, Takahashi, J
• Format: Journal Article
• Language: English
• Published: Asian - Australasian Association of Animal Production Societies 01.06.2010
• Subjects: antimicrobial agents; antimicrobial properties; Control; gas emissions; in vitro studies; lactic acid bacteria; Lactobacillus plantarum; Lactococcus lactis; Leuconostoc citreum; methane production; Methanogenesis; methanogens; Methods; nisin; Properties; protease-resistant antimicrobial substances; proteinase inhibitors; proteinases; Rumen fermentation; strain differences; Japan
• ISSN: 1011-2367; 1976-5517
• DOI: 10.5713/ajas.2010.90444

Effects of protease-resistant antimicrobial substances (PRA) produced by Lactobacillus plantarum and Leuconostoc citreum on rumen methanogenesis were examined using the in vitro continuous methane quantification system. Four different strains of lactic acid bacteria, i) Lactococcus lactis ATCC19435 (Control, non-antibacterial substances), ii) Lactococcus lactis NCIMB702054 (Nisin-Z), iii) Lactobacillus plantarum TUA1490L (PRA-1), and iv) Leuconostoc citreum JCM9698 (PRA-2) were individually cultured in GYEKP medium. An 80 ml aliquot of each supernatant was inoculated into phosphate-buffered rumen fluid. PRA-1 remarkably decreased cumulative methane production, though propionate, butyrate and ammonia N decreased. For PRA-2, there were no effects on C[H.sub.4] and C[O.sub.2] production and fermentation characteristics in mixed rumen cultures. The results suggested that PRA-1 reduced the number of methanogens or inhibited utilization of hydrogen in rumen fermentation. (Key Words: Methane Production, Lactic Acid Bacteria, In vitro Fermentation)