Evidence of a new Tomato yellow leaf curl virus in Japan and its detection using PCR

• Published in: Journal of general plant pathology : JGPP Vol. 71; no. 4; pp. 319 - 325
• Main Authors: Ueda, S.(National Agricultural Research Center for Kyushu Okinawa Region, Nishigoshi, Kumamoto (Japan)), Takeuchi, S, Okabayashi, M, Hanada, K, Tomimura, K, Iwanami, T
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Nature B.V 01.08.2005
• Subjects: BIOLOGICAL DIFFERENCES; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; Flowers & plants; GEMINIVIRUS ENROULEMENT JAUNE TOMAT; GEMINIVIRUS RIZADO AMARILLO TOMATE; Genetics; JAPAN; JAPON; Leaves; LYCOPERSICON ESCULENTUM; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PATOGENICIDAD; PCR; Plant diseases; POUVOIR PATHOGENE; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; TOMATO YELLOW LEAF CURL GEMINIVIRUS; Tomatoes; Japan
• ISSN: 1345-2630; 1610-739X
• DOI: 10.1007/s10327-005-0202-7

A new isolate of Tomato yellow leaf curl virus (TYLCV) has been identified from tomato plants in Kochi Prefecture in Japan and designated TYLCV-[Tosa]. The complete nucleotide sequence of the isolate was determined and found to consist of 2781nt. In phylogenetic analyses of entire nucleotide sequences, TYLCV-[Tosa] was delineated as a single branch and was more closely related to TYLCV-[Almeria] than TYLCV isolates Ng, Sz, or Ai reported in Japan, which had spread since 1996. Isolate TYLCV-[Tosa] is suggested to be a newly introduced, novel isolate of TYLCV that dispersed into Kochi Prefecture. In addition, a rapid method using the polymerase chain reaction to separate TYLCV isolates into four genetic groups was established. This method would be useful for reliable diagnosis based on genetic differences among isolates of TYLCV. [PUBLICATION ABSTRACT]