Stimulation of Bone Marrow-Derived and Peritoneal Macrophages by a T Lymphocyte-Derived Hemopoietic Growth Factor, Persisting Cell-Stimulating Factor

• Published in: Blood Vol. 66; no. 4; pp. 859 - 865
• Main Authors: Crapper, Richard M., Vairo, Gino, Hamilton, John A., Clark-Lewis, Ian, Schrader, John W.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.10.1985; The Americain Society of Hematology
• Subjects: Animals; Biological and medical sciences; Bone Marrow Cells; Cell physiology; Escherichia coli - immunology; Fundamental and applied biological sciences. Psychology; Growth Substances - physiology; Hematopoietic Cell Growth Factors; Lipopolysaccharides - immunology; Macrophage Activation - drug effects; Macrophages - immunology; Mice; Mice, Inbred C3H; Mice, Inbred CBA; Molecular and cellular biology; Peritoneal Cavity - cytology; Phagocytosis - drug effects; Responses to growth factors, tumor promotors, other factors; T-Lymphocytes - cytology; Thymidine - metabolism; Vertebrata; Mammalia; Mouse; Growth; Rodentia; T-Lymphocyte; Lymphokine; Biological activity; Growth factor; Macrophage; Peritoneum
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V66.4.859.859

Several lines of evidence indicated that P cell-stimulating factor (PSF), a T lymphocyte-derived lymphokine known to stimulate the growth of hemopoietic stem and progenitor cells, also acted on macrophages. PSF was absorbed from medium that had been mixed for two hours at 0 °C with either resident or thioglycollate-elicited peritoneal cells, suggesting the presence of receptors for PSF on cells in the population. The addition of pure PSF to populations highly enriched in either resident or elicited adherent peritoneal macrophages resulted in stimulation of macrophages with morphological changes, including increases in size, spreading, vacuolation, and the number of cytoplasmic processes. together with stimulation of proliferation and the phagocytosis of opsonized yeast. PSF also stimulated the incorporation of [sH]thymidine by bone marrow-derived adherent macrophages. Addition of pure PSF to cultures that contained only a single macrophage resulted in enhanced survival and proliferation of these isolated cells, demonstrating that the effect of PSF on macrophages was direct. These results indicate that PSF can stimulate well-differ-entiated functional macrophages and raise the possibility that the effects of PSF on macrophages may play a regulatory role in immune responses. © 1985 by Grune & Stratton, Inc.