Profile of Fecal Microflora in Relation to Food Habit of Male Adult from Pekanbaru, Indonesia

• Published in: IOP conference series. Earth and environmental science Vol. 1460; no. 1; pp. 12053 - 12062
• Main Authors: Pato, Usman, Yusmarini, Riftyan, Emma, Akbar, Wafiatul, Marsoit, Yiohana Enzelina, Agrina, Usman, Aqila Sakura, Suyanto
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.02.2025
• Subjects: adult; Bacteria; Bifidobacterium; Composition; Diet; Digestive system; E coli; Eating; Eating behavior; Fecal microflora; Feces; food habit; Food intake; Habits; Lactic acid; Lactic acid bacteria; male; Microbiota; Microflora; microflora profile; Microorganisms; Multivariate analysis; Pekanbaru; Salmonella; Shigella; Statistical analysis; Variance analysis; Indonesia
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/1460/1/012053

The composition of fecal microflora indicates its digestive system and eating habits. The precise composition can differ significantly based on a person’s diet. This study examined the relationship between 30 adult men’s dietary habits and their fecal microbiota composition. Secondary data were obtained through questionnaires filled out by the subjects. Primary data, including total plate count (TPC), lactic acid bacteria (LAB), Bifidobacterium spp., E. coli , Salmonella spp., and Shigella spp., were analyzed using specific media. The number of microorganisms was analyzed using the ANOVA procedure of SPSS Version 26. The least significant difference procedure was used to determine whether a statistically significant difference existed between the means. Multivariate analysis was conducted to assess the effect of eating habits on the number of fecal microbes. The quantity of microflora, including TPC, LAB, Bifidobacterium spp., E. coli , Salmonella spp., and Shigella spp., varied significantly among participants, according to ANOVA. There were 10.10–12.86 log CFU/g of TPC, 7.72–10.16 log CFU/g of LAB, 3.98–7.42 log CFU/g of Bifidobacterium spp., 7.73–10.16 log CFU/g of E. coli , and 5.29–10.80 log CFU/g of Salmonella spp. and Shigella spp. The study’s findings also revealed that, despite suspicions, all of the bacteria may impact the quantity of TPC, E. coli , Salmonella spp., and Shigella spp., no particular food intake category had an effect. Regarding the individuals’ diet of fruits, vegetables, and nuts, there was a correlation between the variation in LAB and Bifidobacterium counts.