Effects of dietary Lactobacillus helveticus ATC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout (Oncorhynchus mykiss) under high rearing density

• Published in: Annals of animal science Vol. 23; no. 3; pp. 833 - 844
• Main Authors: Jasim, Saade Abdalkareem, Altalbawy, Farag M.A., Alameri, Ameer A., Ramírez-Coronel, Andrés Alexis, Obaid, Rasha Fadhel, Al-Hamdani, Mais Mazin, Kadhim, Abed J., Zabibah, Rahman S., Alzahrani, Hayat Ali, Farsani, Sadegh Ghafari, Dadras, Mahnaz
• Format: Journal Article
• Language: English
• Published: Kraków Sciendo 01.07.2023; De Gruyter Poland
• Subjects: antioxidant; Antioxidants; Bacteria; Catalase; Density; Diet; Digestive enzymes; Erythrocytes; Feed additives; Fish; Globulins; Hematocrit; Hematology; Immune response; Immune system; immunity; Innate immunity; intensive culture; Intestinal microflora; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Lactic acid bacteria; Lactobacilli; Lactobacillus helveticus; Leukocytes (neutrophilic); Lymphocytes; Lysozyme; Oncorhynchus mykiss; Parameters; Peroxidase; probiotic; Probiotics; Respiratory burst; Salmon; Superoxide dismutase; Trout
• ISSN: 2300-8733; 1642-3402; 2300-8733
• DOI: 10.2478/aoas-2023-0056

This study aimed to evaluate the effects of dietary probiotic supplementation of on growth, digestive enzymes, and hematological, biochemical, immune, and antioxidant parameters, as well as intestinal microbiota of rainbow trout ( ). Fish (35.46±0.9 g) were fed with different levels of dietary : control, 1 × 10 , 1 × 10 , 1 × 10 , and 1 × 10 at high stocking density (80 kg m ) for 60 days. Results indicated that growth performance significantly improved in probiotic supplemented fish (P<0.05). Digestive enzyme parameters revealed that supplementation could significantly increase amylase, protease, and lipase (P<0.05). The treated groups showed significant improvements in serum immune parameters including lysozyme (LYZ), alternative complement (ACH ), respiratory burst activity (RBA), and myeloperoxidase (MPO) (P<0.05). Total protein (TP), albumin (ALB), and globulin (GLO) increased in fish fed experimental diets (P<0.05). Lactate dehydrogenase (LDH) activity was significantly lower in fish fed dietary additives (P<0.05) while white blood cells (WBC), lymphocytes, neutrophils, hematocrit (Hct), red blood cells (RBC) were significantly enhanced (P<0.05). Fish fed with supplemented diets showed significantly enhanced antioxidant status, catalase (CAT) and superoxide dismutase (SOD). Malondialdehyde (MDA) content was significantly lower in fish fed dietary additives (P<0.05). Lactic acid bacteria (LAB) in the treatment groups were significantly increased (P<0.05). In conclusion, dietary supplementation of reduced detrimental effects of high stocking density on growth performance and immune response. It appears that can be recommended as a beneficial probiotic feed additive for rainbow trout.