Androgen Inactivation in Human Lung Fibroblasts: Variations in Levels of 17β-Hydroxysteroid Dehydrogenase Type 2 and 5α-Reductase Activity Compatible with Androgen Inactivation

• Published in: The journal of clinical endocrinology and metabolism Vol. 87; no. 8; pp. 3883 - 3892
• Main Authors: Provost, Pierre R., Blomquist, Charles H., Drolet, Renée, Flamand, Nicolas, Tremblay, Yves
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Oxford University Press 01.08.2002; Copyright by The Endocrine Society; Endocrine Society
• Subjects: 17-Hydroxysteroid Dehydrogenases - genetics; 17-Hydroxysteroid Dehydrogenases - metabolism; 3-Hydroxysteroid Dehydrogenases - genetics; 3-Hydroxysteroid Dehydrogenases - metabolism; 3-Oxo-5-alpha-Steroid 4-Dehydrogenase - genetics; 3-Oxo-5-alpha-Steroid 4-Dehydrogenase - metabolism; Androgens; Androgens - metabolism; Androstenedione; Androstenedione - metabolism; Biological and medical sciences; Cell lines; Cells, Cultured; Cytosol - enzymology; Dehydrogenases; Dihydrotestosterone; Dihydrotestosterone - metabolism; Diploids; Embryology: invertebrates and vertebrates. Teratology; Enzyme Activation - physiology; Estradiol Dehydrogenases; Experimental organogenesis; Fibroblasts; Fibroblasts - cytology; Fibroblasts - enzymology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic - physiology; Humans; Lung - cytology; Lungs; Medical sciences; Metabolism; Microsomes - enzymology; mRNA; Organogenesis. Physiological fonctions; Pneumology; Respiratory system : syndromes and miscellaneous diseases; RNA, Messenger - analysis; Steroid 5α-reductase; Substrate preferences; Testosterone; Testosterone - metabolism; Variation; Human; Cell culture; Androgen; Respiratory disease; Enzyme; Pathogenesis; Lung; Metabolism; Ripening; Fetal development; Enzymatic activity; 3(or 17)β-Hydroxysteroid dehydrogenase; Respiratory distress; Oxidoreductases; Sex steroid hormone; Fibroblast; Reductase
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/jcem.87.8.8764

Androgens delay lung maturation through their action on lung fibroblasts. Knowing that testosterone is secreted by the lung epithelial-like cell line A-549, we have studied the metabolism of androgens by several human lung diploid fibroblasts cell lines. No 17-ketosteroid reductase activity was detected. In contrast, testosterone was transformed mainly into androstenedione and androstanedione with no 5α-dihydrotestosterone formed, indicating the presence of 17β- hydroxysteroid dehydrogenase (HSD) type 2 and 5α-reductase activities. The eight cell lines analyzed had either a low or high 17β-HSD type 2 activity level. No correlation between these levels and the sex or age stage of cells was established, but Northern blot analysis of human lung RNA samples of five adult subjects revealed very similar variations between subjects in the level of 17β-HSD type 2 mRNA. The 5α-reductase activity had a marked substrate preference for androstenedione, the product of 17β-HSD type 2. When tritiated testosterone was used as substrate, only barely detectable levels of 5α-dihydrotestosterone were observed by HPLC in the presence of the 17β-HSD type 2 inhibitor EM-919. The use of unlabeled testosterone or of the antiandrogen hydroxyflutamide demonstrated that the tritiated testosterone substrate itself had no effect on levels of 5α-reduction. In fact, in these cells, 5α-reductase has no significant activity on testosterone, but it further converts the product of 17β-HSD type 2, thus playing a role with 17β-HSD type 2 in androgen inactivation. Because androgens delay lung maturation and surfactant synthesis by their action on lung fibroblasts, it is of particular interest to find that the steroid metabolism of these lung fibroblast cells is oriented toward androgen inactivation. Because lung fibroblasts of subjects with low 17β-HSD type 2 expression levels are likely to be exposed to higher levels of androgens, an allelic variation of the 17β-HSD-2 gene is suspected, which would result in familial incidence of respiratory distress. This is in line with reported cases of familial incidence of respiratory distress.