An improved inverse PCR protocol for the generation of T-vectors

T-vectors have been widely used in molecular cloning [1-3]. Com- pared with common vectors, molecular cloning with T-vectors escapes the restriction endonuclease digestion process, decreases the time of DNA purification and thus is time-saving and cost-effective. There are two ways to produce T-vect...

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Published inActa biochimica et biophysica Sinica Vol. 47; no. 2; pp. 142 - 144
Main Authors Chen, Xuejun, Zhang, Hao, Zhang, Yanfang, Cui, Wenyu, Long, Chaoliang, Jia, Xiang, Wang, Hai
Format Journal Article
LanguageEnglish
Published China 01.02.2015
Subjects
Base Sequence
Cloning, Molecular
DNA, Complementary - metabolism
DNA纯化
DNA聚合酶
Electrophoresis, Agar Gel
Endonucleases - metabolism
Escherichia coli - metabolism
Genetic Vectors - genetics
Histones - chemistry
Humans
Molecular Sequence Data
Polymerase Chain Reaction - methods
T-载体
Thymine - metabolism
分子克隆
协议
反向PCR
限制性内切酶
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Summary:T-vectors have been widely used in molecular cloning [1-3]. Com- pared with common vectors, molecular cloning with T-vectors escapes the restriction endonuclease digestion process, decreases the time of DNA purification and thus is time-saving and cost-effective. There are two ways to produce T-vectors： （i） cutting the target vector with EcoRV and then adding a thymine residue to the 3＇-blunt end with Taq DNA polymerase [4].
Bibliography:T-vectors have been widely used in molecular cloning [1-3]. Com- pared with common vectors, molecular cloning with T-vectors escapes the restriction endonuclease digestion process, decreases the time of DNA purification and thus is time-saving and cost-effective. There are two ways to produce T-vectors： （i） cutting the target vector with EcoRV and then adding a thymine residue to the 3＇-blunt end with Taq DNA polymerase [4].
31-1940/Q
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1672-9145
1745-7270
DOI:10.1093/abbs/gmu118