Citrinin mycotoxicosis in the rat. II. Clinicopathological observations

• Published in: Food and cosmetics toxicology Vol. 16; no. 5; pp. 441 - 447
• Main Authors: Jordan, W.H., Carlton, W.W., Sansing, G.A.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.10.1978
• Subjects: Animals; Benzopyrans - toxicity; Citrinin - toxicity; Kidney Diseases - blood; Kidney Diseases - chemically induced; Kidney Diseases - physiopathology; Kidney Diseases - urine; Rats
• ISSN: 0015-6264
• DOI: 10.1016/S0015-6264(78)80302-2

Sprague-Dawley rats weighing about 200 g were given either a single ip dose of 50 mg citrinin/kg or the dimethylsulphoxide-50% ethanol vehicle alone in a volume of 5 ml/kg and several clinicopathological parameters were examined. Alterations found during the first 48 hr after administration of citrinin were indicative of nephrosis. Concentrations of glucose and blood were increased in the urine of citrinin-treated rats and the concentrations were greatest 3 hr after treatment. Protein in the urine increased from a mean of less than 2 + in untreated rats to 3 + 48 hr after citrinin treatment (solvent control = 1 +). A decrease in protein concentration in the urine of solvent-control rats was due to diuresis induced by the dimethylsulphoxide-ethanol solvent. The mean hourly excretions of chloride and sodium in the urine of citrinin-treated rats were 1·5-2·0 times those of solvent-control rats, while potassium excretion was only slightly greater (101 compared with 92 mequiv/litre) in the citrinin-treated than in the solvent-control rats. Activities of glutamic-oxalacetic transaminase and lactic dehydrogenase in the urine of citrinin-treated rats were 15-20 times those of solvent control rats 36 hr after treatment. The blood urea nitrogen increased to 114 mg/100 ml 48 hr after treatment, about five times the normal concentration, Activities of lactic dehydrogenase and glutamic-oxalacetic transaminase in the serum were only slightly elevated in citrinin-treated rats. Serum potassium concentration was elevated and serum sodium and chloride concentrations were reduced.